Morshed M G, Konishi H, Terada Y, Arimitsu Y, Nakazawa T
Department of Microbiology, Yamaguchi University School of Medicine, Japan.
Epidemiol Infect. 1994 Jun;112(3):527-31. doi: 10.1017/s0950268800051220.
Leptospirosis is a worldwide zoonotic disease. In the present investigation, a total of 89 human sera from a flood prone district of Bangladesh was screened by a one-point microscapsule agglutination test (MCAT). MCAT-positive and -doubtful sera were further tested by microscopic agglutination test (MAT) against 16 reference serovars of Leptospira interrogans, and the antibody titres determined. In MCAT, 34 sera were positive and 22 were doubtful. Among those positive and doubtful sera, 33 and 20, respectively were tested by MAT. Thirty-four out of 53 MCAT-screened samples were MAT-positive. The titres ranged from 20 to 1600 with antibodies to serovars copenhageni, australis, cynopteri and icterohaemorrhagiae being the most prevalent. Eleven MCAT-positive samples failed to react with any strains used by MAT, suggesting the presence of new or untested serovars. Among the MAT-positive samples, the presence of antibody against two or more serovars was more common than that of a single serovar. The present study suggests that rural people in Bangladesh are at high risk to leptospiral infection.
钩端螺旋体病是一种全球性人畜共患病。在本调查中，通过单点微胶囊凝集试验（MCAT）对来自孟加拉国一个易受洪水影响地区的89份人类血清进行了筛查。MCAT呈阳性和可疑的血清进一步通过显微镜凝集试验（MAT）针对问号钩端螺旋体的16个参考血清型进行检测，并测定抗体滴度。在MCAT中，34份血清呈阳性，22份可疑。在这些阳性和可疑血清中，分别有33份和20份通过MAT进行检测。在53份经MCAT筛查的样本中，有34份MAT呈阳性。滴度范围为20至1600，其中针对哥本哈根血清型、澳洲血清型、犬血清型和出血性黄疸血清型的抗体最为普遍。11份MCAT阳性样本未能与MAT使用的任何菌株发生反应，表明存在新的或未经检测的血清型。在MAT阳性样本中，存在针对两种或更多血清型的抗体比单一血清型更为常见。本研究表明，孟加拉国农村人口感染钩端螺旋体的风险很高。
