HALPERN B N, LIACOPOULOS P, LIACOPOULOS-BRIOT M, BINAGHI R, VAN NEER F
Immunology. 1959 Oct;2(4):351-62.
Anaphylactic sensitization of guinea pig ileum has been obtained by incubation of the isolated organ in a bath of Tyrode solution containing rabbit antibodies against hen egg albumin. The degree of sensitization has been estimated by the intensity of the contraction of the tissue after addition of the specific antigen. It has been found that the degree of sensitization is proportional to the concentration of antibody in the incubation mixture. A linear relationship has been encountered between the antibody concentration and the reciprocal of the square of the time of incubation, to obtain equal sensitization of the organ. Diminishing the temperature during the incubation considerably slows down the sensitization process. The value of the Q is about 2.5 in the region of 38° to 30°, and 1.6 between 30° and 10°. The sensitization process is not affected by the acidity of the medium, between 8.2 and 5.6, unless carbon dioxide is present, in which case the sensitization is diminished as the H is lowered. Non-specific rabbit gamma-globulin interferes with sensitization. When its concentration is 100-fold that of the antibody, sensitization does not take place. Repeated washing of an already passively sensitized organ does not impair its anaphylactic response. But desensitization can be achieved by incubation with non-specific rabbit gamma-globulin. Treatment with carbonic acid at H 5.8 during one hour significantly affects the anaphylactic condition.
将豚鼠回肠置于含有抗鸡卵白蛋白兔抗体的台氏液浴中孵育，可实现过敏致敏。致敏程度通过加入特异性抗原后组织收缩的强度来评估。已发现致敏程度与孵育混合物中抗体的浓度成正比。为使器官获得同等程度的致敏，抗体浓度与孵育时间平方的倒数之间呈线性关系。孵育期间降低温度会显著减缓致敏过程。在38°至30°区域，Q值约为2.5，在30°至10°之间为1.6。除非存在二氧化碳，否则在8.2至5.6的介质酸度范围内，致敏过程不受影响，若存在二氧化碳，则随着氢离子浓度降低，致敏作用减弱。非特异性兔γ球蛋白会干扰致敏。当其浓度为抗体浓度的100倍时，不会发生致敏。对已被动致敏的器官反复冲洗不会损害其过敏反应。但用非特异性兔γ球蛋白孵育可实现脱敏。在pH 5.8条件下用碳酸处理1小时会显著影响过敏状态。
