Georgiev P G, Elagin V A, Kozitsina M V
Genetika. 1992 Aug;28(8):69-79.
A number of super-unstable systems were obtained earlier by the induction of P-M hybrid dysgenesis in the strains with a mobilized Stalker. One of the super-unstable mutations appeared at the yellow locus of Drosophila melanogaster on the background of preexisting y mutation. The latter had been induced by mdg4 insertion into the regulatory region of the yellow locus. The suppressor genes of Hairy wing and modifier of mdg4 are known to encode proteins which bind to the mdg4 enhancer and are involved in the control of its transcription. A large spectrum of yellow alleles was obtained in super-unstable systems which could be recognized by the intensity of pigmentation in twelve different areas of the cuticle. Combining of these alleles with su(Hw) and mod(mdg4) mutations followed by the study of phenotype changes demonstrated that the same regulatory protein may influence expression of the gene in opposite directions.
早些时候，通过在具有可移动Stalker的品系中诱导P-M杂种发育不全，获得了许多超级不稳定系统。其中一个超级不稳定突变出现在黑腹果蝇黄色基因座上，背景是已有的y突变。后者是由mdg4插入黄色基因座的调控区域诱导产生的。已知毛翅抑制基因和mdg4修饰基因编码与mdg4增强子结合并参与其转录控制的蛋白质。在超级不稳定系统中获得了大量黄色等位基因，可通过表皮十二个不同区域的色素沉着强度来识别。将这些等位基因与su(Hw)和mod(mdg4)突变相结合，随后研究表型变化，结果表明相同的调控蛋白可能以相反的方向影响基因的表达。
