Georgiev P G, Gerasimova T I
N.I. Vavilov Institute of General Genetics, USSR Academy of Sciences, Moscow.
Mol Gen Genet. 1989 Dec;220(1):121-6. doi: 10.1007/BF00260865.
We used a system with a mobilized Stalker transposable element, sometimes in combination with P-M hybrid dysgenesis, in the search for new mutations interfering with the y2 mutation induced by mdg4 (gypsy) insertion into the yellow locus. A novel gene, modifier of mdg4, was detected in chromosome 3. The mutation mod(mdg4) either enhanced or suppressed phenotypic changes in different mutations induced by mdg4 insertions. Thus, mod(mdg4) seems to be involved in the control of mdg4 expression. Six other loci designated as enhancers of yellow were also detected. The e(y)n (with n from 1-6) mutations enhanced the expression of several y mutations induced by different insertions into the yellow locus. The major change is a damage of bristle and hair pigmentation which is not suppressed by su(Hw) mutations. On the other hand, e(y)n alleles do not interact with mdg4 induced mutations in other loci. All e(y)n genes are located in different regions of the X chromosome. One may speculate that e(y)n genes are involved in trans-regulation of the yellow locus and possibly of some other loci.
我们使用了一个带有可移动的Stalker转座元件的系统，有时还结合P-M杂种不育，来寻找干扰由mdg4（gypsy）插入黄色基因座所诱导的y2突变的新突变。在3号染色体上检测到一个新基因，mdg4的修饰基因。mod（mdg4）突变要么增强要么抑制由mdg4插入所诱导的不同突变中的表型变化。因此，mod（mdg4）似乎参与了mdg4表达的调控。还检测到另外六个被指定为黄色增强子的基因座。e（y）n（n从1到6）突变增强了由不同插入黄色基因座所诱导的几种y突变的表达。主要变化是刚毛和毛发色素沉着受损，这不会被su（Hw）突变所抑制。另一方面，e（y）n等位基因与其他基因座中mdg4诱导的突变不相互作用。所有e（y）n基因都位于X染色体的不同区域。可以推测，e（y）n基因参与了黄色基因座以及可能其他一些基因座的反式调控。
