Stark Ken D, Park Eek J, Holub Bruce J
Laboratory of Membrane Biochemistry and Biophysics, Division of Intramural Clinical and Biological Research, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Rockville, MD, USA.
Menopause. 2003 Sep-Oct;10(5):448-55. doi: 10.1097/01.GME.0000059861.93639.1A.
To compare the fatty acid composition of serum phospholipid of premenopausal women with that of postmenopausal women receiving and not receiving hormone replacement therapy (HRT).
Women between the ages of 43 and 70 were recruited for two separate case-comparison studies. Participants were grouped as either premenopausal, postmenopausal receiving HRT, or postmenopausal not receiving HRT. All participants were required to complete a 3-day dietary record before providing a 12-h fasting blood sample. Fatty acid composition of phospholipids and lipid concentrations was determined from serum samples.
The postmenopausal women receiving HRT had significantly higher concentrations of palmitic acid (16:0), palmitoleic acid (16:1), and di-homo-gamma-linolenic (20:3n-6) and significantly lower levels of docosapentaenoic acid (22:5n-3) than the other groups in both studies. In addition, the postmenopausal women receiving HRT had lower levels of behenic (22:0), lignoceric (24:0), and nervonic acid (24:1) in comparison with the postmenopausal women not receiving HRT.
The results of this study indicate that the fatty acid composition of serum phospholipids in women is influenced by menopausal status and hormone use. These results are of interest because high levels of 20:3n-6 and low levels of docosapentaenoic acid have been associated with increased myocardial infarction plus stroke mortality from cardiovascular disease.
比较绝经前女性与接受和未接受激素替代疗法（HRT）的绝经后女性血清磷脂的脂肪酸组成。
招募43至70岁的女性参与两项独立的病例对照研究。参与者被分为绝经前、接受HRT的绝经后或未接受HRT的绝经后三组。所有参与者在提供12小时空腹血样前都需要完成一份为期3天的饮食记录。从血清样本中测定磷脂的脂肪酸组成和脂质浓度。
在两项研究中，接受HRT的绝经后女性的棕榈酸（16:0）、棕榈油酸（16:1）、二高-γ-亚麻酸（20:3n-6）浓度均显著高于其他组，而二十二碳五烯酸（22:5n-3）水平则显著低于其他组。此外，与未接受HRT的绝经后女性相比，接受HRT的绝经后女性的山嵛酸（22:0）、木蜡酸（24:0）和神经酸（24:1）水平较低。
本研究结果表明，女性血清磷脂的脂肪酸组成受绝经状态和激素使用的影响。这些结果之所以令人关注，是因为高水平的20:3n-6和低水平的二十二碳五烯酸与心血管疾病导致的心肌梗死和中风死亡率增加有关。
