Weaver M L, Tanzer J M, Kramer P A
Pharmacokinetics Laboratory, University of Connecticut, Storrs 06268.
J Chromatogr. 1992 Oct 23;581(2):293-6. doi: 10.1016/0378-4347(92)80285-x.
A high-performance liquid chromatographic procedure requiring neither derivatization nor complex sample work-up is reported for reproducibly and sensitively determining pilocarpine in plasma. Following stabilization of pilocarpine against in vitro hydrolysis using sodium fluoride, plasma samples were extracted and the extracts chromatographed on a 5-microns, low-carbon-load (6%) C18 reversed-phase column. The assay was linear between 10 and 300 ng/ml (r = 0.998). It had sufficient sensitivity to quantitate pilocarpine at concentrations as low as 10 ng/ml (signal-to-noise ratio > or = 4) using a 500-microliters sample. The assay appears to be the first published specifically for plasma determinations and has proven capable of supporting pharmacokinetics studies of pilocarpine disposition in the anesthetized dog.
本文报道了一种高效液相色谱法，该方法无需衍生化处理，也无需复杂的样品预处理，可用于可重复且灵敏地测定血浆中的毛果芸香碱。使用氟化钠使毛果芸香碱在体外水解稳定后，提取血浆样品，提取物在5微米、低碳负载（6%）的C18反相柱上进行色谱分析。该测定法在10至300纳克/毫升之间呈线性（r = 0.998）。使用500微升样品时，它具有足够的灵敏度，能够定量低至10纳克/毫升浓度的毛果芸香碱（信噪比≥4）。该测定法似乎是首次专门针对血浆测定而发表的，并且已证明能够支持对麻醉犬体内毛果芸香碱处置的药代动力学研究。
