Pfützner A, Dietrich U, von Eichel U, von Briesen H, Brede H D, Maniar J K, Rübsamen-Waigmann H
Chemotherapeutisches Forschungsinstitut, Georg-Speyer-Haus (GSH), Frankfurt, Germany.
J Acquir Immune Defic Syndr (1988). 1992 Oct;5(10):972-7.
A high-risk population (patients of a sexually transmitted disease clinic and the GT hospital in Bombay) was tested for antibodies against HIV-1 and HIV-2. Among 405 serum samples, 226 had previously been classified HIV-positive in India using different locally available enzyme-linked immunosorbent assay (ELISA) tests. The serology of 179 samples was unknown. All 405 samples were tested at the Georg-Speyer-Haus (GSH) with the Pasteur HIV-1/2-Combi-ELISA. Positive samples were further analyzed with HIV-1 and HIV-2 Western blot kits from Dupont and Pasteur, respectively. A very high seroprevalence of HIV was found in this population. Among the 179 unscreened samples, 69 (38.5%) were positive in the ELISAs as well as the Western blots for HIV-1 or HIV-2. Among the prescreened samples, only 174 (77%) were confirmed HIV-positive. Altogether, 243 of 405 sera were HIV-positive. Of these, 184 (76%) were reactive with HIV-1, 10 (4%) were reactive with HIV-2, and 49 (20%) had dual reactivity to HIV-1 and HIV-2. Previous data from the Indian Council of Medical Research had already suggested a possible high prevalence of HIV-1 in India. Our results confirm this view. The finding of a substantial spread of HIV-2 infection was, however, totally unexpected in India, but confirms our previous study which had already demonstrated the existence of HIV-2 in this country. Asia can thus no longer be considered free of HIV-2, and testing for HIV-2 appears mandatory, at least in India.(ABSTRACT TRUNCATED AT 250 WORDS)
对高危人群（一家性传播疾病诊所的患者以及孟买 GT 医院的患者）进行了抗 HIV-1 和 HIV-2 抗体检测。在 405 份血清样本中，有 226 份此前在印度使用不同的本地可用酶联免疫吸附测定（ELISA）检测被分类为 HIV 阳性。179 份样本的血清学情况未知。所有 405 份样本均在格奥尔格 - 施派尔豪斯（GSH）使用巴斯德 HIV-1/2 联合 ELISA 进行检测。阳性样本分别使用杜邦和巴斯德的 HIV-1 和 HIV-2 免疫印迹试剂盒进一步分析。在该人群中发现 HIV 的血清阳性率非常高。在 179 份未筛查样本中，69 份（38.5%）在 ELISA 以及针对 HIV-1 或 HIV-2 的免疫印迹检测中呈阳性。在预先筛查的样本中，只有 174 份（77%）被确认为 HIV 阳性。405 份血清样本中共有 243 份为 HIV 阳性。其中有 184 份（76%）与 HIV-1 反应，10 份（4%）与 HIV-2 反应，49 份（20%）对 HIV-1 和 HIV-2 具有双重反应性。印度医学研究理事会之前的数据已经表明印度可能存在 HIV-1 的高流行率。我们的结果证实了这一观点。然而，HIV-2 感染大量传播这一发现，在印度完全出乎意料，但证实了我们之前的研究，该研究已证明该国存在 HIV-2。因此，亚洲不能再被认为没有 HIV-2，至少在印度，对 HIV-2 的检测似乎是必要的。（摘要截取自 250 字）
