Kumar C G, Takagi H
Dairy Microbiology Division, National Dairy Research Institute, Karnal 132 001, India.
Biotechnol Adv. 1999 Dec 15;17(7):561-94. doi: 10.1016/s0734-9750(99)00027-0.
Alkaline proteases are of considerable interest in view of their activity and stability at alkaline pH. This review describes the proteases that can resist extreme alkaline environments produced by a wide range of alkalophilic microorganisms. Different isolation methods are discussed which enable the screening and selection of promising organisms for industrial production. Further, strain improvement using mutagenesis and/or recombinant DNA technology can be applied to augment the efficiency of the producer strain to a commercial status. The various nutritional and environmental parameters affecting the production of alkaline proteases are delineated. The purification and properties of these proteases is discussed, and the use of alkaline proteases in diverse industrial applications is highlighted.
鉴于碱性蛋白酶在碱性pH值下的活性和稳定性，它们备受关注。本综述描述了能够抵抗多种嗜碱微生物产生的极端碱性环境的蛋白酶。讨论了不同的分离方法，这些方法有助于筛选和选择有工业生产潜力的微生物。此外，可以应用诱变和/或重组DNA技术来改良菌株，以提高生产菌株的效率使其达到商业水平。阐述了影响碱性蛋白酶生产的各种营养和环境参数。讨论了这些蛋白酶的纯化和性质，并着重介绍了碱性蛋白酶在各种工业应用中的用途。
