Lu G, DeLisle A J, de Vetten N C, Ferl R J
Horticultural Sciences Department, University of Florida, Gainesville.
Proc Natl Acad Sci U S A. 1992 Dec 1;89(23):11490-4. doi: 10.1073/pnas.89.23.11490.
The G box is a well-characterized cis-acting DNA regulatory element found in the promoters of several seemingly unrelated plant genes, including the alcohol dehydrogenase (Adh) gene of Arabidopsis thaliana. Using a monoclonal antibody screening approach coupled with electrophoretic mobility shift assays, we have isolated a cDNA clone encoding a protein that is part of the in vitro protein/G box complex. The derived amino acid sequence is homologous to a class of proteins in mammalian brains described as protein kinase C inhibitors and as activators of tyrosine and tryptophan hydroxylases, the rate-limiting enzymes in the pathways leading to the catecholamines and serotonin. The fact that a homologous member of this regulatory protein family is found in plants and is associated with binding to transcriptional regulatory elements suggests a much wider role for these proteins.
G盒是一种特征明确的顺式作用DNA调控元件，存在于几种看似不相关的植物基因的启动子中，包括拟南芥的乙醇脱氢酶（Adh）基因。通过将单克隆抗体筛选方法与电泳迁移率变动分析相结合，我们分离出一个编码蛋白质的cDNA克隆，该蛋白质是体外蛋白质/G盒复合物的一部分。推导的氨基酸序列与哺乳动物大脑中的一类蛋白质同源，这类蛋白质被描述为蛋白激酶C抑制剂以及酪氨酸和色氨酸羟化酶的激活剂，而酪氨酸和色氨酸羟化酶是通往儿茶酚胺和5-羟色胺途径中的限速酶。在植物中发现这个调控蛋白家族的一个同源成员并与转录调控元件结合，这一事实表明这些蛋白质具有更广泛的作用。
