脂肪组织和骨髓多谱系细胞上干细胞动员相关分子的差异表达

Differential expression of stem cell mobilization-associated molecules on multi-lineage cells from adipose tissue and bone marrow.

作者信息

De Ugarte Daniel A, Alfonso Zeni, Zuk Patricia A, Elbarbary Amir, Zhu Min, Ashjian Peter, Benhaim Prosper, Hedrick Mare H, Fraser John K

机构信息

Department of Surgery, UCLA School of Medicine, Regenerative Bioengineering and Repair Laboratory, Los Angeles, CA, USA.

出版信息

Immunol Lett. 2003 Oct 31;89(2-3):267-70. doi: 10.1016/s0165-2478(03)00108-1.

DOI:10.1016/s0165-2478(03)00108-1

PMID:14556988

Abstract

Our laboratory has characterized a population of stromal cells obtained from adipose tissue termed processed lipoaspirate cells (PLAs). PLAs, like bone-marrow derived mesenchymal stem cells (BM-MSCs), have the capacity to differentiate along the adipogenic, osteogenic, chondrogenic, and myogenic lineages, In order to better characterize these two multi-lineage populations, we examined the surface phenotype of both bone marrow and adipose tissue-derived cells from five patients undergoing surgery. PLA and BM-MSC cells were isolated, subcultivated, and evaluated for cell surface marker expression using flow cytometry. PLA and BM-MSC cells both expressed CD13, CD29, CD44, CD90, CD105, SH-3, and STRO-1. Differences in expression were noted for cell adhesion molecules CD49d (Integrin alpha4), CD54 (ICAM-1), CD34, and CD106 (VCAM-1). While markedly similar, the surface phenotypes of PLA and BM-MSC cells are distinct for several cell adhesion molecules implicated in hematopoietic stem cell homing, mobilization, and proliferation.

摘要

我们实验室已对从脂肪组织中获取的一群基质细胞进行了特性分析，这些细胞被称为处理过的脂肪抽吸物细胞（PLA）。PLA与骨髓来源的间充质干细胞（BM-MSC）一样，具有沿脂肪生成、成骨、软骨生成和肌生成谱系分化的能力。为了更好地描述这两个多谱系细胞群体，我们检测了五名接受手术患者的骨髓和脂肪组织来源细胞的表面表型。分离、传代培养PLA和BM-MSC细胞，并使用流式细胞术评估细胞表面标志物的表达。PLA和BM-MSC细胞均表达CD13、CD29、CD44、CD90、CD105、SH-3和STRO-1。在细胞黏附分子CD49d（整合素α4）、CD54（细胞间黏附分子-1）、CD34和CD106（血管细胞黏附分子-1）的表达上发现了差异。虽然PLA和BM-MSC细胞的表面表型明显相似，但在一些与造血干细胞归巢、动员和增殖有关的细胞黏附分子方面却有所不同。