Chaigneau Emmanuelle, Oheim Martin, Audinat Etienne, Charpak Serge
Laboratory of Neurophysiology, INSERM EPI 0002, Centre National de la Recherche Scientifique FRE2500, Ecole Supérieure de Physique et Chimie Industrielles de la Ville de Paris, 10 Rue Vauquelin, 75231 Paris, France.
Proc Natl Acad Sci U S A. 2003 Oct 28;100(22):13081-6. doi: 10.1073/pnas.2133652100. Epub 2003 Oct 20.
Analysis of the spatiotemporal coupling between neuronal activity and cerebral blood flow requires the precise measurement of the dynamics of RBC flow in individual capillaries that irrigate activated neurons. Here, we use two-photon microscopy in vivo to image individual RBCs in glomerular capillaries in the rat dorsal olfactory bulb. We find that odor stimulation evokes capillary vascular responses that are odorant- and glomerulus-specific. These responses consist of increases as well as decreases in RBC flow, both resulting from independent changes in RBC velocity or linear density. Finally, measuring RBC flow with micrometer spatial resolution and millisecond temporal resolution, we demonstrate that, in olfactory bulb superficial layers, capillary vascular responses precisely outline regions of synaptic activation.
分析神经元活动与脑血流之间的时空耦合需要精确测量为激活神经元供血的单个毛细血管中红细胞流动的动力学。在这里，我们使用体内双光子显微镜对大鼠背侧嗅球中肾小球毛细血管内的单个红细胞进行成像。我们发现气味刺激会引发特定于气味剂和肾小球的毛细血管血管反应。这些反应包括红细胞流动的增加和减少，两者均由红细胞速度或线密度的独立变化引起。最后，我们以微米级的空间分辨率和毫秒级的时间分辨率测量红细胞流动，证明在嗅球浅层，毛细血管血管反应精确勾勒出突触激活区域。
