Kulka Ulrike, Schaffer Moshe, Siefert Axel, Schaffer Pamela M, Olsner Astrid, Kasseb Kawser, Hofstetter Alfons, Dühmke Eckhart, Jori Giulio
Department of Radiation Therapy, University of Munich, Munich, Germany.
Biochem Biophys Res Commun. 2003 Nov 7;311(1):98-103. doi: 10.1016/j.bbrc.2003.09.170.
Chemical modifiers (radiosensitizers) are used in order to increase the efficacy of radiotherapy. The use of Photodynamic Therapy for tumor treatment, especially with Photofrin II, is also known. At present, no chemical modifier has been found to act as a selective radiosensitizer. Experiments using several series of cell lines were performed; human bladder cancer cell line (RT4), colon adenocarcinoma cells (HT-29), and the glioblastoma cells (U-373 MG) were investigated, with and without incubation with Photofrin II, before irradiation. The irradiation was performed using doses ranging from 0 to 8Gy. Colony forming tests were applied to determine the efficiency of Photofrin II as a radiation sensitizer in comparison to irradiation alone. Two of the cell lines tested, cultures of the RT4 and U-373 MG, treated with Photofrin II prior to radiation, showed cell survival lower than cultures untreated with Photofrin II but irradiated under identical conditions. For the HT-29 cells, the results did not differ between the two groups (with and without Photofrin). The results of this study showed that Photofrin II can act, under certain conditions as a tumor radiosensitizer.
为提高放射治疗的疗效，人们使用化学修饰剂（放射增敏剂）。光动力疗法用于肿瘤治疗，尤其是使用卟吩姆钠（Photofrin II）进行治疗也为人所知。目前，尚未发现有化学修饰剂可作为选择性放射增敏剂。进行了一系列使用多种细胞系的实验；研究了人膀胱癌细胞系（RT4）、结肠腺癌细胞（HT - 29）和胶质母细胞瘤细胞（U - 373 MG），在照射前，对其进行卟吩姆钠（Photofrin II）孵育或不孵育的处理。照射剂量范围为0至8Gy。应用集落形成试验来确定与单独照射相比，卟吩姆钠（Photofrin II）作为放射增敏剂的效果。所测试的两种细胞系，即RT4和U - 373 MG的培养物，在辐射前用卟吩姆钠（Photofrin II）处理，其细胞存活率低于未用卟吩姆钠（Photofrin II）处理但在相同条件下照射的培养物。对于HT - 29细胞，两组（使用和未使用卟吩姆钠）的结果没有差异。本研究结果表明，在某些条件下，卟吩姆钠（Photofrin II）可作为肿瘤放射增敏剂。
