酿酒酵母中ADH4基因的自发扩增
Spontaneous amplification of the ADH4 gene in Saccharomyces cerevisiae.
作者信息
Dorsey M, Peterson C, Bray K, Paquin C E
机构信息
Department of Biological Sciences, University of Cincinnati, Ohio 45221-0006.
出版信息
Genetics. 1992 Dec;132(4):943-50. doi: 10.1093/genetics/132.4.943.
Abstract
Five spontaneous amplifications of the ADH4 gene were identified among 1,894 antimycin A-resistant mutants isolated from a diploid strain after growth at 15 degrees. Four of these amplifications are approximately 40-kb linear extrachromosomal palindromes carrying telomere homologous sequences at each end similar to a previously isolated amplification. ADH4 is located at the extreme left end of chromosome VII, and the extrachromosomal fragments appear to be the fusion of two copies of the end of this chromosome. The fifth amplification is a chromosomal amplification carrying an extra copy of ADH4 on both homologs of chromosome VII. These results suggest that the ADH system can be used to study amplification in Saccharomyces cerevisiae.
摘要
在从一个二倍体菌株分离出的1894个抗抗霉素A突变体中，于15摄氏度生长后鉴定出5个ADH4基因的自发扩增。其中4个扩增是大约40kb的线性染色体外回文序列，两端带有端粒同源序列，类似于先前分离出的扩增序列。ADH4位于染色体VII的最左端，染色体外片段似乎是该染色体末端两个拷贝的融合。第五个扩增是染色体扩增，在染色体VII的两个同源染色体上都携带一个额外的ADH4拷贝。这些结果表明，ADH系统可用于研究酿酒酵母中的扩增。
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