Samaranayake Y H, Samaranayake L P, Yau J Y Y, Ellepola A N B, Anil S, Yeung K W S
Oral Biosciences, Faculty of Dentistry, University of Hong Kong, Hong Kong.
Mycoses. 2003;46(9-10):375-83. doi: 10.1046/j.0933-7407.2003.00919.x.
Objectives of the study were to investigate the variability in yeast adhesion and cell-surface-hydrophobicity (CSH) during human immunodeficiency virus (HIV) disease progression, using a total of 60 sequential Candida albicans isolated from oral rinse samples of seven HIV-infected individuals with (4) and without (3) clinical symptoms of oropharyngeal candidosis. Significant differences in the adhesion to buccal epithelial cells (BECs) during sequential visits were observed for all genetic isotypes in five of the seven individuals and three isotypes belonging to the sixth individual. A single isotype of patient HK1 and another of HK4 (genotype I) demonstrated significant variations in their CSH during sequential visits whereas no such differences were noted for the remaining genotypes. On Spearman correlation analysis an isotype from HK1 demonstrated a significant increased adherence to BECs and CSH during HIV disease progression whereas no such correlation was noted for the remaining isotypes studied. No significant differences in adherence to BECs or CSH values were observed between the symptomatic oral candidosis and the asymptomatic carrier group. Further, on regression analysis only the single isotype of HK1 demonstrated a significant positive correlation between adherence to BECs and CSH whereas no such correlation was observed when all tested Candida isolates were pooled and evaluated as a single, large group.
本研究的目的是，使用从7名感染人类免疫缺陷病毒（HIV）的个体（其中4名有口腔念珠菌病临床症状，3名没有）的口腔冲洗样本中分离出的60株白色念珠菌，调查HIV疾病进展过程中酵母黏附性和细胞表面疏水性（CSH）的变异性。在7名个体中的5名以及第6名个体的3种基因同型中，观察到所有基因同型在连续访视期间对颊黏膜上皮细胞（BEC）的黏附存在显著差异。患者HK1的一种同型和HK4（基因型I）的另一种同型在连续访视期间其CSH表现出显著变化，而其余基因型未观察到此类差异。在Spearman相关性分析中，HK1的一种同型在HIV疾病进展期间对BEC的黏附性和CSH显著增加，而其余研究的同型未观察到此类相关性。有症状的口腔念珠菌病组和无症状携带者组之间，在对BEC的黏附性或CSH值方面未观察到显著差异。此外，在回归分析中，只有HK1的单一同型在对BEC的黏附性和CSH之间表现出显著正相关，而当将所有测试的念珠菌分离株合并作为一个大组进行评估时，未观察到此类相关性。
