Sweet S P, Cookson S, Challacombe S J
Department of Oral Medicine and Pathology, UMDS Guy's Hospital, London.
J Med Microbiol. 1995 Dec;43(6):452-7. doi: 10.1099/00222615-43-6-452.
The increased prevalence of oral candidosis associated with HIV infection must be intrinsically related to immunological changes in the host, but might also involve alterations to the infecting strains of yeast. This study aimed to determine if strains of Candida albicans isolated from asymptomatic HIV-infected individuals or AIDS patients possessed altered adherence properties in an in-vitro buccal epithelial cell (BEC) adherence assay. C. albicans isolates from 49 patients with HIV infection or AIDS adhered to BEC in significantly higher numbers than isolates from 49 control subjects (p < 0.001). No significant differences in adherence were detected between strains isolated from HIV-infected or AIDS subjects, or between strains isolated from C. Albicans carriers (low salivary C. albicans counts) or subjects with oral candidosis. The presence of whole saliva significantly inhibited the binding of candida to BEC (p < 0.001), but the significant difference in adherence between the HIV/AIDS and control isolates was maintained. The effect of saliva was independent of salivary candida antibodies and was abolished by treatment with protease or neuraminidase, suggesting the involvement of salivary mucins. The results of this study suggest that HIV infection is associated with the selection of strains of C. albicans with and increased ability to adhere to oral mucosa.
与HIV感染相关的口腔念珠菌病患病率增加必然与宿主的免疫变化内在相关，但也可能涉及感染酵母菌株的改变。本研究旨在确定从无症状HIV感染者或艾滋病患者中分离出的白色念珠菌菌株在体外颊上皮细胞（BEC）黏附试验中是否具有改变的黏附特性。49例HIV感染或艾滋病患者的白色念珠菌分离株黏附于BEC的数量明显高于49例对照受试者的分离株（p < 0.001）。从HIV感染或艾滋病受试者中分离出的菌株之间，或从白色念珠菌携带者（唾液中白色念珠菌计数低）或口腔念珠菌病患者中分离出的菌株之间，未检测到黏附的显著差异。全唾液的存在显著抑制念珠菌与BEC的结合（p < 0.001），但HIV/艾滋病患者和对照分离株之间黏附的显著差异仍然存在。唾液的作用独立于唾液念珠菌抗体，并且用蛋白酶或神经氨酸酶处理后作用消失，提示唾液黏蛋白参与其中。本研究结果表明，HIV感染与选择具有增加的黏附口腔黏膜能力的白色念珠菌菌株有关。
