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穆氏文殊兰培养物中的生物碱生成

Alkaloid production in Crinum moorei cultures.

作者信息

Fennell C W, Elgorashi E E, van Staden J

机构信息

Research Centre for Plant Growth and Development, School of Botany and Zoology, Faculty of Science and Agriculture, University of Natal Pietermaritzburg, Private Bag X01, Scottsville, 3209, South Africa.

出版信息

J Nat Prod. 2003 Nov;66(11):1524-6. doi: 10.1021/np030206o.

DOI:10.1021/np030206o
PMID:14640535
Abstract

The alkaloids cherylline (1), crinamidine (2), crinine (3), epibuphanisine (4), lycorine (5), powelline (6), undulatine (7), 1-epideacetylbowdensine (8), and 3-O-acetylhamayne (9) were identified in the in vitro propagated bulblets of Crinum moorei. In addition, crinine, powelline, and undulatine were detected in the solidified Murashige and Skoog (MS) medium. The identity of the alkaloids was confirmed by comparing retention times and mass spectra with known samples. Light, as well as benzyladenine (BA) and charcoal supplementation of the tissue culture medium, influenced the levels of specific alkaloids in both the bulblets and media.

摘要

在体外繁殖的穆氏文殊兰小鳞茎中鉴定出了生物碱

秋水仙灵(1)、海葱脒(2)、海葱碱(3)、表布法辛(4)、石蒜碱(5)、鲍威林(6)、波状碱(7)、1-表去乙酰博登辛(8)和3-O-乙酰哈马因(9)。此外,在固化的Murashige和Skoog(MS)培养基中检测到了海葱碱、鲍威林和波状碱。通过将保留时间和质谱与已知样品进行比较,确认了生物碱的身份。光照以及组织培养基中添加苄基腺嘌呤(BA)和活性炭,均影响了小鳞茎和培养基中特定生物碱的含量。

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