van den Eijnde S M, Boshart L, Baehrecke E H, De Zeeuw C I, Reutelingsperger C P, Vermeij-Keers C
MGC Department of Clinical Genetics, Erasmus University Medical School, Rotterdam, The Netherlands.
Apoptosis. 1998;3(1):9-16. doi: 10.1023/a:1009650917818.
Exposure of the aminophospholipid phosphatidylserine at the outer leaflet of the plasma membrane by apoptotic cells can trigger phagocytic removal of these dying cells. This functionality of phosphatidylserine exposure in the process of phagocytosis is indicated by in vitro studies of mammalian and insect phagocytes. We have studied the in vivo distribution of cell-surface exposed phosphatidylserine by injecting biotinylated Annexin V, a Ca2+ -dependent phosphatidyl-serine binding protein, into viable mouse and chick embryos and Drosophila pupae. The apparent binding of Annexin V to cells with a morphology which is characteristic of apoptosis and which was present in regions of developmental cell death indicates that phosphatidylserine exposure by apoptotic cells is a phylogenetically conserved mechanism.
凋亡细胞使质膜外小叶上的氨基磷脂磷脂酰丝氨酸暴露，可触发对这些垂死细胞的吞噬清除。哺乳动物和昆虫吞噬细胞的体外研究表明了磷脂酰丝氨酸暴露在吞噬过程中的这一功能。我们通过将生物素化的膜联蛋白V（一种Ca2+依赖的磷脂丝氨酸结合蛋白）注射到存活的小鼠、鸡胚胎和果蝇蛹中，研究了细胞表面暴露的磷脂酰丝氨酸在体内的分布。膜联蛋白V与具有凋亡特征形态且存在于发育性细胞死亡区域的细胞的明显结合表明，凋亡细胞使磷脂酰丝氨酸暴露是一种系统发育保守机制。
