Seto E, Usheva A, Zambetti G P, Momand J, Horikoshi N, Weinmann R, Levine A J, Shenk T
Howard Hughes Medical Institute, Princeton University, NJ 08544-1014.
Proc Natl Acad Sci U S A. 1992 Dec 15;89(24):12028-32. doi: 10.1073/pnas.89.24.12028.
p53 activates transcription of genes with a p53 response element, and it can repress genes lacking the element. Here we demonstrate that wild-type but not mutant p53 inhibits transcription in a HeLa nuclear extract from minimal promoters. Wild-type but not mutant p53 binds to human TATA-binding protein (TBP). p53 does not bind to yeast TBP, and it cannot inhibit transcription in a HeLa extract where yeast TBP substitutes for human TBP. These results suggest a model in which p53 binds to TBP and interferes with transcriptional initiation.
p53可激活带有p53反应元件的基因转录，并且能够抑制缺乏该元件的基因。在此我们证明，野生型而非突变型p53在HeLa细胞核提取物中可抑制最小启动子的转录。野生型而非突变型p53可与人TATA结合蛋白（TBP）结合。p53不与酵母TBP结合，并且在酵母TBP替代人TBP的HeLa提取物中，它无法抑制转录。这些结果提示了一种模型，即p53与TBP结合并干扰转录起始。
