南瓜韧皮部凝集素基因在伴胞中特异性表达。
Pumpkin phloem lectin genes are specifically expressed in companion cells.
作者信息
Bostwick D E, Dannenhoffer J M, Skaggs M I, Lister R M, Larkins B A, Thompson G A
机构信息
Department of Plant Sciences, University of Arizona, Tucson 85721.
出版信息
Plant Cell. 1992 Dec;4(12):1539-48. doi: 10.1105/tpc.4.12.1539.
Abstract
Pumpkin phloem exudate contains two abundant phloem proteins: PP1 is a 96-kD protein that forms polymeric filaments in vivo, and PP2 is a 48-kD dimeric lectin. Polyclonal antibodies raised against pumpkin phloem exudate were used to isolate several cDNAs corresponding to PP1 and PP2. RNA gel blot analysis indicated that PP1 is encoded by an mRNA of approximately 2500 nucleotides, whereas PP2 subunits are encoded by an mRNA of 1000 nucleotides. Sequence analysis of PP2 cDNAs revealed a 654-bp open reading frame encoding a 218-amino acid polypeptide; this polypeptide had the carbohydrate binding characteristics of a PP2 subunit. The PP2 mRNA was localized within the phloem of pumpkin hypocotyl cross-sections based on in situ hybridization of a digoxigenin-labeled antisense probe. PP2 mRNA was found within the companion cells in both the bicollateral vascular bundles and the extrafascicular phloem network.
摘要
南瓜韧皮部渗出液含有两种丰富的韧皮部蛋白
PP1是一种96-kD的蛋白，在体内形成聚合物细丝，PP2是一种48-kD的二聚体凝集素。针对南瓜韧皮部渗出液产生的多克隆抗体被用于分离几个与PP1和PP2对应的cDNA。RNA凝胶印迹分析表明，PP1由一个约2500个核苷酸的mRNA编码，而PP2亚基由一个1000个核苷酸的mRNA编码。PP2 cDNA的序列分析揭示了一个654-bp的开放阅读框，编码一个218个氨基酸的多肽；该多肽具有PP2亚基的碳水化合物结合特性。基于地高辛标记的反义探针的原位杂交，PP2 mRNA定位于南瓜下胚轴横切面的韧皮部内。在双韧维管束和束间韧皮部网络的伴胞中均发现了PP2 mRNA。
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