Tonon Sergio Andrés, Picconi María Alejandra, Zinovich Jorge Bruno, Nardari Wanda, Mampaey Mariana, Badano Inés, Di Lello Federico, Galuppo Juan Antonio, Alonio Lidia Virginia, Teyssie Angélica Rita
Laboratorio de Biologi;a Molecular Aplicada, Universidad Nacional de Misiones, Félix de Azara 1552, Posadas, Misiones-3300, Argentina.
Int J Infect Dis. 2004 Jan;8(1):13-9. doi: 10.1016/j.ijid.2003.03.001.
To evaluate the prevalence of human papillomavirus (HPV) cervical infection in women from the South American Guarani Indian tribe located in the rain forest of Misiones, north-eastern Argentina; a region with a high incidence of cervical carcinoma.
A cross-sectional cytological and HPV screening of sexually active Guarani women from nine Indian settlements was conducted. Demographic data, information about sexual behavior, and gynaecological history were recorded. Fresh cervical specimens from 239 patients were collected, of which 207 were included in this study. Cytology and microbiological detection were carried out by the Papanicolaou and Gram stain methods, respectively. HPV detection and typing were analyzed by PCR and RFLP.
Pap smears in 96% of all patients showed an inflammatory pattern. A possible etiologic agent was found in 58% of cases: 52% Trichomonas vaginalis, 35% Gardnerella vaginalis and 13% Candida sp. Seven cases had cytological changes compatible with Low Grade Intraepithelial Lesion (LGSIL), one with High Grade Intraepithelial Lesion (HGSIL) and one in situ cervical cancer. The prevalence for generic HPV infection was 64% (133/207). Genotyping gave a 26% prevalence for HPV types 16/18, 13% for types 6/11 and 30% for other types, with nine mixed infections.
This work reports for the first time the prevalence of cervical HPV infection in Guarani women. Nearly all Guarani women had some grade of cervical disease. Generic HPV infection prevalence was elevated (64%), with predominance of high risk types 16/18. A large variety of viral types was detected, including high to intermediate risk types not found previously in the region.
评估位于阿根廷东北部米西奥内斯雨林中的南美瓜拉尼印第安部落女性人乳头瘤病毒（HPV）宫颈感染的患病率；该地区宫颈癌发病率较高。
对来自九个印第安定居点的性活跃瓜拉尼女性进行了横断面细胞学和HPV筛查。记录了人口统计学数据、性行为信息和妇科病史。收集了239例患者的新鲜宫颈标本，其中207例纳入本研究。细胞学和微生物学检测分别采用巴氏染色法和革兰氏染色法。通过PCR和RFLP分析HPV检测和分型。
所有患者中96%的巴氏涂片显示为炎症模式。58%的病例发现了可能的病原体：52%为阴道毛滴虫，35%为阴道加德纳菌，13%为念珠菌属。7例有与低级别上皮内瘤变（LGSIL）相符的细胞学改变，1例为高级别上皮内瘤变（HGSIL），1例为宫颈原位癌。HPV总体感染率为64%（133/207）。基因分型显示HPV 16/18型的感染率为26%，6/11型为13%，其他类型为30%，有9例混合感染。
本研究首次报告了瓜拉尼女性宫颈HPV感染的患病率。几乎所有瓜拉尼女性都有某种程度的宫颈疾病。HPV总体感染率较高（64%），高危型16/18为主。检测到多种病毒类型，包括该地区此前未发现的高风险至中风险类型。
