Agren M S, Taplin C J, Woessner J F, Eaglstein W H, Mertz P M
Department of Dermatology and Cutaneous Surgery, University of Miami School of Medicine, Florida 33101.
J Invest Dermatol. 1992 Dec;99(6):709-14. doi: 10.1111/1523-1747.ep12614202.
Collagenase is believed to be important for cell migration and collagen remodeling during tissue repair and regeneration. We have investigated collagenase concentrations in different types of surgically inflicted wounds in pigs. Collagenase was extracted from tissue homogenates of wounds by heating to 60 degrees C for 6 min in 0.1 M CaCl2. The molecular weight of latent collagenase was about 52 kDa. Activated collagenase produced the characteristic 3/4 fragment of collagen. Collagenase was assayed by the use of radiolabeled telopeptide-free collagen. To detect maximal collagenase activity, extracts were reduced and alkylated to destroy inhibitors, then activated with aminophenylmercuric acetate. Sutured incisions showed peak collagenase content on postoperative day 1 and thereafter steadily declining concentrations. Granulation tissue from non-sutured large defect full-thickness wounds showed high collagenase content on postoperative day 5 and then a sharp decline to day 7 followed by a slowly declining curve to postoperative day 21. Partial-thickness wounds exhibited a different time course, with collagenase increasing to peak concentrations on postoperative days 3-5; however, a large proportion of the detected collagenase was due to the adherent scab. By day 7 collagenase concentrations approached the low concentrations of normal skin when epithelialization was complete and the scab rejected. In general, collagenase shows an early maximum and then declines with postoperative time, with the sharpest decline occurring when epithelialization is complete.
胶原酶被认为在组织修复和再生过程中对细胞迁移和胶原重塑很重要。我们研究了猪不同类型手术伤口中的胶原酶浓度。通过在0.1M氯化钙中加热至60摄氏度6分钟从伤口组织匀浆中提取胶原酶。潜在胶原酶的分子量约为52kDa。活化的胶原酶产生特征性的胶原3/4片段。通过使用放射性标记的无端肽胶原测定胶原酶。为检测最大胶原酶活性，提取物经还原和烷基化以破坏抑制剂，然后用乙酸氨基苯汞活化。缝合切口在术后第1天显示胶原酶含量峰值，此后浓度稳步下降。非缝合的大缺损全层伤口的肉芽组织在术后第5天显示高胶原酶含量，然后到第7天急剧下降，随后到术后第21天呈缓慢下降曲线。部分厚度伤口呈现不同的时间进程，胶原酶在术后第3 - 5天增加到峰值浓度；然而，检测到的大部分胶原酶归因于附着的痂皮。到第7天，当上皮化完成且痂皮脱落时，胶原酶浓度接近正常皮肤的低浓度。一般来说，胶原酶显示早期最大值，然后随术后时间下降，上皮化完成时下降最明显。
