Iwatsuki Kenji, Akihisa Toshihiro, Tokuda Harukuni, Ukiya Motohiko, Oshikubo Manabu, Kimura Yumiko, Asano Takeshi, Nomura Atsushi, Nishino Hoyoku
K-Laboratories for Intelligent Medical Remote Services, 2266-22 Anagahora, Shimoshidami, Moriyama-ku, Nagoya 463-0003, Japan.
J Nat Prod. 2003 Dec;66(12):1582-5. doi: 10.1021/np0302293.
A new triterpene acid, lucidenic acid P (1a), and two new triterpene acid methyl esters, methyl lucidenates P (1b) and Q (2b), were isolated and characterized from the fruiting body of the fungus Ganoderma lucidum. Their structures were elucidated on the basis of spectroscopic methods. In addition, eight known triterpene acids, lucidenic acids A (3a), C (4a), D(2) (5a), E(2) (6a), and F (7a) and ganoderic acids E (9a), F (10a), and T-Q (11a), and six known triterpene acid methyl esters, methyl lucidenates A (3b), D(2) (5b), E(2) (6b), F (7b), and L (8b) and methyl ganoderate F (10b), were isolated and identified from the fungus. All of the triterpenoids, with the exception of 7a, were evaluated with respect to their inhibitory effects on the induction of Epstein-Barr virus early antigen (EBV-EA) by 12-O-tetradecanoylphorbol-13-acetate (TPA) in Raji cells, which is known to be a primary screening test for antitumor promoters. All of the compounds tested showed potent inhibitory effects on EBV-EA induction (96-100% inhibition at 1 x 10(3) mol ratio/TPA).
从灵芝子实体中分离并鉴定出一种新的三萜酸——灵芝酸P（1a）以及两种新的三萜酸甲酯——灵芝酸甲酯P（1b）和Q（2b）。通过光谱方法阐明了它们的结构。此外，还从该真菌中分离并鉴定出8种已知的三萜酸，即灵芝酸A（3a）、C（4a）、D(2)（5a）、E(2)（6a）、F（7a）以及灵芝酸E（9a）、F（10a）和T-Q（11a），还有6种已知的三萜酸甲酯，即灵芝酸甲酯A（3b）、D(2)（5b）、E(2)（6b）、F（7b）、L（8b）以及灵芝酸甲酯F（10b）。除7a外，所有三萜类化合物均针对其对12-O-十四酰佛波醇-13-乙酸酯（TPA）诱导Raji细胞中Epstein-Barr病毒早期抗原（EBV-EA）的抑制作用进行了评估，这是一种已知的抗肿瘤促进剂的初步筛选试验。所有测试化合物均对EBV-EA诱导显示出强效抑制作用（在1×10(3)摩尔比/TPA时抑制率为96 - 100%）。
