Holschneider D P, Maarek J-M I, Yang J, Harimoto J, Scremin O U
Department of Psychiatry and the Behavioral Sciences, University of Southern California, Los Angeles, CA, USA.
Neurosci Lett. 2004 Jan 2;354(1):74-8. doi: 10.1016/j.neulet.2003.09.078.
Most brain mapping techniques require immobilization of the subject, which extinguishes all but the simplest behaviors. We applied in freely moving rats an implantable microbolus infusion pump (MIP) which can be triggered by remote activation for the injection of the cerebral blood flow tracer [(14)C]iodoantipyrine during behavioral activation. Consistent with previous electrophysiological, metabolic and brain anatomic studies, CBF-related tissue radioactivity (CBF-TR) increased in acoustic cortex during a 1000 Hz/8000 Hz alternating tone. In response to an acute foot-shock, CBF-TR increased in visual cortex, parietal association cortex, and extended into primary motor cortex, and primary somatosensory cortex mapping the trunk. These results support the utility of implantable pumps as adjunct tools for studying cerebral activation during behavioral challenges in nontethered, nonrestrained animals.
大多数脑图谱技术都需要使受试者保持固定，这会消除除最简单行为之外的所有行为。我们在自由活动的大鼠身上应用了一种可植入的微量注射泵（MIP），该泵可通过远程激活触发，以便在行为激活期间注射脑血流示踪剂[（14）C]碘安替比林。与先前的电生理、代谢和脑解剖学研究一致，在1000 Hz/8000 Hz交替音调期间，听觉皮层中与脑血流量相关的组织放射性（CBF-TR）增加。响应急性足部电击，视觉皮层、顶叶联合皮层中的CBF-TR增加，并延伸至初级运动皮层以及映射躯干的初级体感皮层。这些结果支持了可植入泵作为辅助工具在未系绳、未受限动物行为挑战期间研究脑激活的实用性。
