传统用于治疗癌症的泰国药用植物的体外细胞毒性活性。

In vitro cytotoxic activity of Thai medicinal plants used traditionally to treat cancer.

作者信息

Itharat Arunporn, Houghton Peter J, Eno-Amooquaye E, Burke P J, Sampson Julia H, Raman Amala

机构信息

Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Science, Prince of Songkhla University, Hat-Yai, Songkhla 90110, Thailand.

出版信息

J Ethnopharmacol. 2004 Jan;90(1):33-8. doi: 10.1016/j.jep.2003.09.014.

DOI:10.1016/j.jep.2003.09.014

PMID:14698505

Abstract

The SRB assay was used to test cytotoxicity against three human cancer cell lines and one normal cell line of 11 Thai medicinal plant species used by traditional doctors in treating cancer patients. The extraction procedures used were similar to those practised by Thai traditional doctors (ethanolic and water extracts). Extracts were tested against the human large cell lung carcinoma cell line COR-L23, the human breast adenocarcinoma cell line MCF-7 and human colon adenocarcinoma cell line LS-174T and normal human keratinocytes SVK-14. The results showed that three plants; Dioscorea membranacea Pierre ex Prain & Burkill, Dioscorea birmanica Prain & Burkill (Dioscoreaceae) and Siphonodon celastrineus Griff. (Celastraceae), exhibited high cytotoxic activity showing a certain degree of selectivity against the different cell types.

摘要

采用SRB法检测了泰国传统医生用于治疗癌症患者的11种泰国药用植物对三种人类癌细胞系和一种正常细胞系的细胞毒性。所采用的提取程序与泰国传统医生的做法类似（乙醇提取物和水提取物）。提取物针对人类大细胞肺癌细胞系COR-L23、人类乳腺腺癌细胞系MCF-7、人类结肠腺癌细胞系LS-174T和正常人角质形成细胞SVK-14进行了测试。结果表明，三种植物，即薯蓣科的膜叶薯蓣Pierre ex Prain & Burkill、缅甸薯蓣Prain & Burkill和卫矛科的密花藤Siphonodon celastrineus Griff.，表现出高细胞毒性活性，对不同细胞类型显示出一定程度的选择性。