Ottander Ulrika, Solensten Nils Gunnar, Bergh Anders, Olofsson Jan I
Departments of Clinical Science/Obstetrics and Gynecology, Umeå University, Umeå, Sweden.
Fertil Steril. 2004 Jan;81(1):154-9. doi: 10.1016/j.fertnstert.2003.05.019.
To evaluate the morphologic characteristics underlying the ultrasonographic appearance and blood flow dynamics in the human corpus luteum (CL) of the menstrual cycle.
Cross-sectional study.
Umeå University Hospital, Umeå, Sweden.
PATIENT(S): Twenty-six otherwise healthy women with proven fertility and a history of regular menstrual cycles, scheduled for elective hysterectomy or tubal sterilization.
INTERVENTION(S): An ovulatory LH rise in urine was established and the CL age was determined according to the day after the LH rise. Before surgery, a standardized ultrasonographic examination of the CL, including B-mode and color Doppler ultrasonography measurements, was performed. Upon commencing the minilaparotomy, the CL was excised and measured using a digital slide-caliper. The volume density (percentage of CL volume occupied by blood vessels) of factor VIII-related antigen immunostained endothelial cells was determined.
MAIN OUTCOME MEASURE(S): Pulsatility index obtained from intraovarian blood vessels supplying the CL and volume density of blood vessels in CL tissue. The CL maximal and minimal outer and inner dimensions were measured in vivo by ultrasonography and ex vivo by a digital slide caliper.
RESULT(S): A statistically significant decrease of blood vessel density and an increased resistance to blood flow, as indicated by pulsatility index, was established during the course of corpus luteum development. An inverse correlation between pulsatility index and volume density of blood vessels was found. A high degree of agreement between ultrasonographic and anatomic measurements of surgically removed CL was found.
CONCLUSION(S): Transvaginal ultrasonography in combination with intraovarian color Doppler flow measurements is a simple and reliable method to evaluate the size and vascularization of the human CL.
评估月经周期中人类黄体（CL）超声表现及血流动力学的形态学特征。
横断面研究。
瑞典于默奥大学医院，于默奥。
26名身体健康、生育能力正常且月经周期规律、计划行择期子宫切除术或输卵管绝育术的女性。
确定尿中促黄体生成素（LH）排卵峰出现，并根据LH峰出现后的天数确定黄体年龄。手术前，对黄体进行标准化超声检查，包括B型超声和彩色多普勒超声测量。开始小切口剖腹手术时，切除黄体并用数字游标卡尺测量。测定因子VIII相关抗原免疫染色内皮细胞的体积密度（血管占黄体体积的百分比）。
供应黄体的卵巢内血管的搏动指数以及黄体组织中血管的体积密度。通过超声在体内测量黄体的最大和最小外径及内径，通过数字游标卡尺在体外测量。
在黄体发育过程中，血管密度有统计学意义的下降，搏动指数表明血流阻力增加。发现搏动指数与血管体积密度呈负相关。手术切除的黄体超声测量与解剖测量之间有高度一致性。
经阴道超声联合卵巢内彩色多普勒血流测量是评估人类黄体大小和血管化的一种简单可靠的方法。
