Arizono T, Oga M, Sugioka Y
Department of Orthopedics, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Acta Orthop Scand. 1992 Dec;63(6):661-4. doi: 10.1080/17453679209169731.
The pathobiology of total joint prosthesis infection was investigated in vitro. Discs of polymethylmethacrylate (PMMA) were exposed to a suspension containing cells of 10(8) per mL Staphylococcus epidermidis E-46. After 12 hours, exposed discs were rinsed with phosphate-buffered saline and placed in brain heart infusion broth containing antibiotics (2.5 mg per mL of Cephaloridine). After gentle shaking for 24 hours at 37 degrees C, the bacteria on the PMMA surface were detached and washed with phosphate-buffered saline to remove the antibiotics. Compared with the free bacteria which were detached from the PMMA by sonication immediately after exposure to the antibiotic solution, those allowed to remain adhered to the PMMA surface were more resistant to antibiotics. Scanning electron microscopy showed accumulation of bacteria surrounded by slime on PMMA discs exposed for 12 hours. Our results indicate that resistance of bacteria to antibiotics is increased after adherence to the biomaterial and formation of a slime layer.
在体外研究了全关节假体感染的病理生物学。将聚甲基丙烯酸甲酯（PMMA）圆盘暴露于每毫升含10(8)表皮葡萄球菌E - 46细胞的悬液中。12小时后，将暴露的圆盘用磷酸盐缓冲盐水冲洗，然后置于含抗生素（每毫升2.5毫克头孢菌素）的脑心浸液肉汤中。在37℃轻轻振荡24小时后，将PMMA表面的细菌分离并用磷酸盐缓冲盐水洗涤以去除抗生素。与暴露于抗生素溶液后立即通过超声处理从PMMA分离的游离细菌相比，那些仍附着在PMMA表面的细菌对抗生素更具抗性。扫描电子显微镜显示，暴露12小时的PMMA圆盘上有被黏液包围的细菌聚集。我们的结果表明，细菌附着于生物材料并形成黏液层后，其对抗生素的抗性会增加。
