卡皮利亚结核分枝杆菌阴性菌株中编码MPB64蛋白的基因发生包括IS6110插入在内的突变。
Mutations including IS6110 insertion in the gene encoding the MPB64 protein of Capilia TB-negative Mycobacterium tuberculosis isolates.
作者信息
Hirano Kazue, Aono Akio, Takahashi Mitsuyoshi, Abe Chiyoji
机构信息
Department of Basic Research, Research Institute of Tuberculosis, Fukujuji Hospital, Kiyose-shi, Tokyo 204-8533, Japan.
出版信息
J Clin Microbiol. 2004 Jan;42(1):390-2. doi: 10.1128/JCM.42.1.390-392.2004.
Abstract
A simple immunochromatographic assay, Capilia TB, using anti-MPB64 monoclonal antibodies, is a kit for discriminating between the Mycobacterium tuberculosis complex and mycobacteria other than tubercle bacilli. The sensitivity of the kit was estimated to be 99.2% (381 of 384 samples). The sequencing analysis revealed that all of the Capilia TB-negative isolates had mutations within the mpb64 gene, leading to the production of an incomplete protein as a result of a deletion of the C-terminal region of the protein.
摘要
一种简单的免疫层析检测方法Capilia TB，使用抗MPB64单克隆抗体，是一种用于区分结核分枝杆菌复合群和非结核分枝杆菌的试剂盒。该试剂盒的灵敏度估计为99.2%（384个样本中的381个）。测序分析表明，所有Capilia TB阴性分离株的mpb64基因均发生突变，导致该蛋白C末端区域缺失，从而产生不完全蛋白。
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