Thrane U
Department of Biotechnology, Technical University of Denmark, Lyngby, Denmark.
Int J Food Microbiol. 1996 Apr;29(2-3):149-56. doi: 10.1016/0168-1605(95)00040-2.
At the Second International Workshop on Standardisation of Methods for the Mycological Examination of Foods in Baarn, 1990, three selective media for food-borne Fusarium species were recommended: Czapek-Dox Iprodione Dichloran Agar (CZID), Dichloran Chloramphenicol Peptone agar (DCPA) and Pentachloronitrobenzene Peptone agar (PPA). Lack of sufficient data made it impossible to recommend one Fusarium selective medium. In the present study nine laboratories from seven countries compared CZID, DCPA, and PPA by analysing 10 samples of flour spiked with conidia of Fusarium avenaceum and F. verticillioides (= F. moniliforme). The colony forming units were counted. The total counts on each of the three media were within a similar range for each participant, and no significant differences between the three media were evident. However, the development of Fusarium colonies was quite different on the three media, and most collaborators found it possible to differentiate these Fusarium species by pigmentation on CZID. Pigmentation was much less conspicuous on PPA, and inconspicuous in DCPA cultures. CZID is recommended as the best currently available selective medium for Fusarium isolates from foods.
1990年在巴恩举行的第二届食品真菌学检验方法标准化国际研讨会上，推荐了三种用于检测食源性镰刀菌属的选择性培养基：查氏-多克斯异菌脲二氯苯胺琼脂（CZID）、二氯苯胺氯霉素蛋白胨琼脂（DCPA）和五氯硝基苯蛋白胨琼脂（PPA）。由于缺乏足够的数据，无法推荐一种单一的镰刀菌选择性培养基。在本研究中，来自七个国家的九个实验室通过分析10份添加了燕麦镰刀菌和轮枝镰刀菌（=串珠镰刀菌）分生孢子的面粉样本，对CZID、DCPA和PPA进行了比较。统计了菌落形成单位。对于每个参与者，三种培养基上的总计数都在相似的范围内，三种培养基之间没有明显的显著差异。然而，三种培养基上镰刀菌菌落的生长情况差异很大，大多数合作者发现可以通过CZID上的色素沉着来区分这些镰刀菌物种。PPA上的色素沉着不太明显，DCPA培养物中则不明显。推荐CZID作为目前从食品中分离镰刀菌的最佳选择性培养基。
