Mentel R, Ilgert U, Wegner U, Zimmerman K, Bruns R, Gürtler L
Friedrich Loeffler Institute of Medical Microbiology, Greifswald, Germany.
Med Microbiol Immunol. 2005 Jan;194(1-2):67-71. doi: 10.1007/s00430-003-0215-9.
The objective of this study was to determine the importance of respiratory syncytial virus (RSV) for hospitalization in the north east of Germany and to obtain molecular epidemiological data of the circulating strains. Using a rapid and sensitive reverse transcriptase-PCR, it was found that a quarter of pediatric respiratory disease admissions were due to RSV. Infections caused by RSV in hospitalized patients were determined over the whole year. Both RSV groups A and B were identified with a predominance of RSV A (86%) over the entire period. The analysis of the deduced amino acid sequences by direct sequencing showed that very similar RSV strains are circulating in the community.
本研究的目的是确定呼吸道合胞病毒（RSV）在德国东北部住院治疗中的重要性，并获取流行毒株的分子流行病学数据。使用快速灵敏的逆转录聚合酶链反应（RT-PCR）发现，四分之一的儿科呼吸道疾病住院病例是由RSV引起的。全年确定了住院患者中由RSV引起的感染情况。RSV A组和B组均被识别出来，在整个时期内RSV A占主导地位（86%）。通过直接测序对推导的氨基酸序列进行分析表明，社区中传播的RSV毒株非常相似。
