Fukunishi K, Murai N, Uno H
Advanced Research Laboratory, Hitachi Ltd., Saitama, Japan.
Biol Cybern. 1992;67(6):501-9. doi: 10.1007/BF00198757.
The spatiotemporal characteristics of neural activity in the guinea pig auditory cortex are investigated to determine their importance in neural processing and coding of the complex sounds. A multi-channel optical recording system has been developed for observing the cortical field of the mammalian brain in vivo. Using the voltage-sensitive dye: RH795, optical imaging was used to visualize neural activity in the guinea pig auditory cortex. Experimental results reveal a boomerang-shaped pattern of movement of activated neural cell regions for the evoked response to click as complex sounds. Parallel and sequential neural processing structure was observed. Although the exact frequency selectivities of single cells and tonotopical organization observed using microelectrode were not visible, the similar feature to the microelectrode evidences was imaged by extracting the strongly response field from the optical data.
研究豚鼠听觉皮层神经活动的时空特征，以确定它们在复杂声音的神经处理和编码中的重要性。已开发出一种多通道光学记录系统，用于在体内观察哺乳动物大脑的皮层区域。使用电压敏感染料RH795，通过光学成像来可视化豚鼠听觉皮层中的神经活动。实验结果揭示了对于作为复杂声音的点击诱发反应，激活的神经细胞区域呈回旋镖状移动模式。观察到了并行和顺序神经处理结构。尽管使用微电极观察到的单个细胞的确切频率选择性和音频拓扑组织不可见，但通过从光学数据中提取强反应场，成像出了与微电极证据相似的特征。
