He Qing-Yu, Chen Jia, Kung Hsiang-Fu, Yuen Anthony Po-Wing, Chiu Jen-Fu
Department of Chemistry, University of Hong Kong, Hong Kong, China.
Proteomics. 2004 Jan;4(1):271-8. doi: 10.1002/pmic.200300550.
Oral tongue carcinoma is an aggressive tumor that particularly affects chronic smokers, drinkers and betel squid chewers. Patients often present symptoms at a late stage, and there is a high recurrence rate after treatment. In this article, we report the first proteomic analysis of oral tongue carcinoma to globally search for tumor related proteins. Apart from helping us to understand the molecular pathogenesis of the carcinoma, these proteins may also have potential clinical applications as biomarkers, enabling the tumor to be identified at an early stage in high risk individuals, treatment response to be predicted, and residual or recurrent carcinoma to be detected sooner after treatment. The protein expression profiles of ten oral tongue squamous cell carcinomas and their matched normal mucosal resection margins were examined by two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization-time of flight mass spectroscopy. A number of tumor-associated proteins including heat shock protein (HSP)60, HSP27, alpha B-crystalline, ATP synthase beta, calgranulin B, myosin, tropomyosin and galectin 1 were consistently found to be significantly altered in their expression levels in tongue carcinoma tissues, compared with their paired normal mucosae. The expression profile portrays a global protein alteration that appears specific to oral tongue cancer. The potential of utilizing these tumor related proteins for screening cancer and monitoring recurrence warrants further investigation.
口腔舌癌是一种侵袭性肿瘤，尤其影响长期吸烟者、饮酒者和嚼槟榔者。患者通常在晚期出现症状，治疗后复发率很高。在本文中，我们报告了首次对口腔舌癌进行的蛋白质组学分析，以全面寻找肿瘤相关蛋白。除了帮助我们了解该癌症的分子发病机制外，这些蛋白作为生物标志物还可能具有潜在的临床应用价值，能够在高危个体中早期识别肿瘤、预测治疗反应，并在治疗后更快地检测出残留或复发性癌症。通过二维凝胶电泳和基质辅助激光解吸/电离飞行时间质谱法检测了10例口腔舌鳞状细胞癌及其匹配的正常黏膜切除边缘的蛋白质表达谱。与配对的正常黏膜相比，在舌癌组织中，包括热休克蛋白（HSP）60、HSP27、αB-晶状体蛋白、ATP合酶β、钙粒蛋白B、肌球蛋白、原肌球蛋白和半乳糖凝集素1在内的许多肿瘤相关蛋白的表达水平持续显著改变。该表达谱描绘了一种似乎特定于口腔舌癌的整体蛋白质改变。利用这些肿瘤相关蛋白进行癌症筛查和监测复发的潜力值得进一步研究。
