Lee Sang Myung, Min Bung Sun, Lee Cheal-Gyu, Kim Kab-Sig, Kho Yung Hee
KT&G Central Research Institute, Yuseong, Daejon, Korea.
Planta Med. 2003 Nov;69(11):1051-4. doi: 10.1055/s-2003-45155.
The following eleven triterpenoic acids were isolated from the fruits of Zizyphus jujuba (Rhamnaceae): colubrinic acid, alphitolic acid, 3-O-cis-p-coumaroylalphitolic acid (3), 3-O-trans-p-coumaroylalphitolic acid (4), 3-O-cis-p-coumaroylmaslinic acid, 3-O-trans-p-coumaroylmaslinic acid, betulinic acid (7), oleanolic acid, betulonic acid (9), oleanonic acid and zizyberenalic acid. The in vitro cytotoxicities of the triterpenoic acids against K562, B16(F-10), SK-MEL-2, PC-3, LOX-IMVI, and A549 tumor cell lines were investigated by the sulforhodamin B (SRB) method. Among these compounds, the lupane-type triterpenes, such as compounds 3, 4, 7, and 9, showed high cytotoxic activities. In particular, the cytotoxic activities of 3-O-p-coumaroylalphitolic acids (compounds 3 and 4) were better than those of non-coumaroic triterpenenoids (compounds 7 and 9). These results suggest that the coumaroyl moiety at the C-3 position of the lupane-type triterpene may play an important role in enhancing cytotoxic activity.
从枣（鼠李科）果实中分离出以下11种三萜酸：蛇麻脂醇酸、阿弗酸、3 - O - 顺式 - 对香豆酰阿弗酸（3）、3 - O - 反式 - 对香豆酰阿弗酸（4）、3 - O - 顺式 - 对香豆酰齐墩果酸、3 - O - 反式 - 对香豆酰齐墩果酸、桦木酸（7）、齐墩果酸、桦木酮酸（9）、齐墩果酮酸和酸枣仁烯酸。采用磺酰罗丹明B（SRB）法研究了这些三萜酸对K562、B16（F - 10）、SK - MEL - 2、PC - 3、LOX - IMVI和A549肿瘤细胞系的体外细胞毒性。在这些化合物中，羽扇豆烷型三萜，如化合物3、4、7和9，表现出高细胞毒性活性。特别是，3 - O - 对香豆酰阿弗酸（化合物3和4）的细胞毒性活性优于非香豆酸三萜类化合物（化合物7和9）。这些结果表明，羽扇豆烷型三萜C - 3位的香豆酰部分可能在增强细胞毒性活性中起重要作用。
