Meyer U, Joos U, Mythili J, Stamm T, Hohoff A, Fillies T, Stratmann U, Wiesmann H P
Department of Cranio-Maxillofacial Surgery, University of Münster, Waldeyerstr. 30, D-48149, Münster, Germany.
Biomaterials. 2004 May;25(10):1959-67. doi: 10.1016/j.biomaterials.2003.08.070.
Primary stability and an optimized load transfer are assumed to account for an undisturbed osseointegration process of implants. Immediate loaded newly designed titanium dental implants inserted in the mandible of minipigs were used for the characterization of the interfacial area between the implant surface and the surrounding bone tissue during the early healing phase. Histological and electron microscopical studies were performed from implant containing bone specimens. Two different load regimens were applied to investigate the load related tissue reaction. Histological and electron microscopical analysis revealed a direct bone apposition on the implant surfaces, as well as the attachment of cells and matrix proteins in the early loading phase. A striking finding of the ultrastructural immunocytochemical investigations was the synthesis and deposition of bone related proteins (osteonectin, fibronectin, fibronectin receptor) by osteoblasts from day one of bone/biomaterial interaction. Calcium-phosphate needle-like crystallites were newly synthesized in a time-related manner directly at the titanium surface. No difference in the ultrastructural appearance of the interface was found between the two loading groups. Our experimental data suggest that loading of specially designed implants can be performed immediately after insertion without disturbing the biological osseointegration process.
初步稳定性和优化的载荷传递被认为是种植体实现无干扰骨结合过程的原因。将新设计的即刻加载钛牙种植体植入小型猪下颌骨，用于表征早期愈合阶段种植体表面与周围骨组织之间的界面区域。对含种植体的骨标本进行了组织学和电子显微镜研究。应用两种不同的加载方案来研究与载荷相关的组织反应。组织学和电子显微镜分析显示，在早期加载阶段，种植体表面有直接的骨附着，以及细胞和基质蛋白的附着。超微结构免疫细胞化学研究的一个显著发现是，从骨/生物材料相互作用的第一天起，成骨细胞就合成并沉积了与骨相关的蛋白质（骨连接蛋白、纤连蛋白、纤连蛋白受体）。磷酸钙针状微晶以与时间相关的方式直接在钛表面新合成。两个加载组之间在界面的超微结构外观上没有发现差异。我们的实验数据表明，特殊设计的种植体在植入后可立即加载，而不会干扰生物骨结合过程。
