Tams S H, Bauer E, Oettler G, Melchinger A E
State Plant Breeding Institute, University of Hohenheim, Fruwirthstrasse 21, 70593 Stuttgart, Germany.
Theor Appl Genet. 2004 May;108(7):1385-91. doi: 10.1007/s00122-003-1552-1. Epub 2004 Feb 4.
Knowledge of the genetic diversity of a species is important for the choice of crossing parents in line and hybrid breeding. Our objective was to investigate European winter triticale using simple sequence repeat (SSR) markers and the coancestry coefficient ( f) with regard to genetic diversity and grouping of germplasm. Three to five primer pairs for each of the 42 chromosomes were selected to analyse 128 European winter triticale varieties and breeding lines. SSR analysis resulted in the identification of 657 alleles with an average of 6.8 alleles per primer pair. The average polymorphism information content (PIC) for polymorphic markers was 0.54. Correlation between f and genetic similarity (GS) estimates based on Rogers' Distance was low (r(fxGS(ABDR))=0.33). The analysis of molecular variance (AMOVA) revealed that 84.7% of the total variation was found within breeding companies, and 15.3% among them. In conclusion, SSR markers from wheat and rye provide a powerful tool for assessing genetic diversity in triticale. Even though no distinct groups within the European winter triticale pool could be detected by principal co-ordinate analysis, this study provides basic information about the genetic relationships for breeding purposes.
了解一个物种的遗传多样性对于品系和杂交育种中亲本的选择很重要。我们的目标是利用简单序列重复（SSR）标记和共祖系数（f）来研究欧洲冬性小黑麦的遗传多样性和种质分组。为42条染色体中的每条选择3至5对引物，以分析128个欧洲冬性小黑麦品种和育种系。SSR分析共鉴定出657个等位基因，每个引物对平均有6.8个等位基因。多态性标记的平均多态信息含量（PIC）为0.54。基于罗杰斯距离的f与遗传相似性（GS）估计值之间的相关性较低（r(fxGS(ABDR)) = 0.33）。分子方差分析（AMOVA）表明，总变异的84.7%存在于育种公司内部，15.3%存在于公司之间。总之，来自小麦和黑麦的SSR标记为评估小黑麦的遗传多样性提供了一个有力工具。尽管通过主坐标分析在欧洲冬性小黑麦群体中未检测到明显的分组，但本研究为育种目的提供了有关遗传关系的基本信息。
