De Clerck Elke, De Vos Paul
Laboratory of Microbiology, Department of Biochemistry, Physiology and Microbiology, Ghent University, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium.
FEMS Microbiol Lett. 2004 Feb 9;231(1):91-8. doi: 10.1016/S0378-1097(03)00935-2.
Bacillus licheniformis is exploited industrially for the production of enzymes and has been shown to exhibit pathogenic properties. Because of these divergent characteristics, questions arise concerning intraspecies diversity. A comparative study by means of combined repetitive polymerase chain reaction, rpoB and gyrA sequencing, 16S rDNA targeted probe analysis, DNA-DNA hybridizations, gelatinase tests and antibiotic susceptibility tests was performed on a set of strains from diverse sources, including strains with pathogenic potential. B. licheniformis was found to consist of two lineages that are distinguished genotypically.
地衣芽孢杆菌在工业上被用于酶的生产,并且已显示出具有致病特性。由于这些不同的特征,关于种内多样性的问题随之而来。通过组合重复聚合酶链反应、rpoB和gyrA测序、16S rDNA靶向探针分析、DNA-DNA杂交、明胶酶测试和抗生素敏感性测试,对一组来自不同来源的菌株进行了比较研究,包括具有致病潜力的菌株。结果发现,地衣芽孢杆菌由两个在基因型上有区别的谱系组成。