Melissas J, Wasas A, Wadee A A, Korkozoglou E, Flessas P
Abteilung für Chirurgie und Mikrobiologie, Medizinische Fakultät, Universität von Witwatersrand, R.S.A.
Langenbecks Arch Chir. 1992;377(6):341-4. doi: 10.1007/BF00574771.
A new rabbit model to study the consequences of splenectomy in host resistance to induced pneumococcal septicaemia is presented. A simple, fast, non-invasive and complication-free technique of bacterial inoculation into the rabbit airways, using transcutaneous cannulation of the trachea, is also described. Sixteen normal and 24 splenectomised animals were used. The optimal dose of pneumonococcus (serotype B, type III) was found to be 1 x 10(7) organisms. The above dose of bacteria given transtracheally failed to cause signs of pneumonia or death in any of the normal rabbits. However the same dose introduced using the same technique resulted reproducibly in the death of the low-resistance splenectomised animals.
本文介绍了一种新的兔模型，用于研究脾切除对宿主抵抗诱导性肺炎球菌败血症的影响。还描述了一种简单、快速、无创且无并发症的将细菌接种到兔气道的技术，即经皮气管插管法。使用了16只正常动物和24只脾切除动物。发现肺炎球菌（B型，III型）的最佳剂量为1×10⁷个菌。经气管给予上述剂量的细菌，在任何正常兔中均未引起肺炎迹象或死亡。然而，使用相同技术给予相同剂量的细菌，可重复性地导致低抵抗力的脾切除动物死亡。
