Jakics E B, Iyobe S, Hirai K, Fukuda H, Hashimoto H
Laboratory of Drug Resistance in Bacteria, School of Medicine, Gunma University, Japan.
Antimicrob Agents Chemother. 1992 Nov;36(11):2562-5. doi: 10.1128/AAC.36.11.2562.
Seven spontaneous norfloxacin (NFLX)-resistant mutants obtained in vitro from 20 NFLX-susceptible clinical isolates and 3 NFLX-resistant clinical isolates of Pseudomonas aeruginosa were transformed with the pNF111 plasmid, whose BamHI fragment is responsible for conferring susceptibility to NFLX, by complementing the nfxB mutation. The resulting patterns of MICs of NFLX, beta-lactams, aminoglycosides, and chloramphenicol and the observed increased accumulation of NFLX were consistent with the occurrence of the nfxB type mutation in these clinical isolates.
从20株对诺氟沙星（NFLX）敏感的铜绿假单胞菌临床分离株和3株对NFLX耐药的临床分离株体外获得的7个自发NFLX耐药突变体，用pNF111质粒进行转化，该质粒的BamHI片段通过互补nfxB突变赋予对NFLX的敏感性。NFLX、β-内酰胺类、氨基糖苷类和氯霉素的最低抑菌浓度（MIC）结果模式以及观察到的NFLX积累增加与这些临床分离株中发生nfxB型突变一致。
