Baranton G, Assous M, Postic D
Unité de Bactériologique moléculaire et médicale, Institut Pasteur, Paris.
Bull Acad Natl Med. 1992 Oct;176(7):1075-85; discussion 1085-6.
DNAs from various Borrelia associated with Lyme disease were reciprocally hybridized. This genomic taxonomy method showed that Lyme disease agent comprised three genomic species. Two species could be differentiated by phenotypic characters as major proteins molecular weights and monoclonal antibodies reactivity. Western-blot with sera from patients suffering from different clinical forms of Lyme disease: arthritis, meningoradiculitis and Acrodermatitis Chronicum Atrophicans showed that each of these evolutive forms was preferentially associated with one of the species, respectively: Borrelia burgdorferi sensu stricto, Borrelia garinii and Borrelia group VS 461. Furthermore, geographical repartition of these three species was heterogeneous. B. burgdorferi sensu stricto seems to be the only one present in the United States of America, whereas it coexists in Europe mainly with B. garinii in Western Europe and Borrelia group VS 461 in Northern Europe.
对与莱姆病相关的各种疏螺旋体的DNA进行了相互杂交。这种基因组分类方法表明，莱姆病病原体包括三个基因组种。其中两个种可通过表型特征（如主要蛋白质分子量和单克隆抗体反应性）加以区分。用患有不同临床形式莱姆病（关节炎、脑脊神经根炎和慢性萎缩性肢端皮炎）患者的血清进行蛋白质免疫印迹分析表明，这些演进形式分别优先与其中一个种相关：狭义伯氏疏螺旋体、伽氏疏螺旋体和VS 461疏螺旋体群。此外，这三个种的地理分布并不均匀。狭义伯氏疏螺旋体似乎是美国唯一存在的种，而在欧洲，它主要与伽氏疏螺旋体在西欧共存，与VS 461疏螺旋体群在北欧共存。
