Holcomb M, Wilson D M, Trucksess M W, Thompson H C
National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR 72079.
J Chromatogr. 1992 Oct 30;624(1-2):341-52. doi: 10.1016/0021-9673(92)85687-o.
Several chromatographic methods for the determination of aflatoxins in agricultural and food products are reviewed. During the past two decades, identification and determination of aflatoxins were done by thin-layer chromatography (TLC) because it was easy, fast and inexpensive. However, high-performance liquid chromatography (HPLC) using fluorescence detection is now the method of choice for determining aflatoxins and is also growing in popularity for their identification. The reasons for selecting HPLC over TLC can be summarized as the ability to analyze for a wide variety of compounds, including compounds that are easily degraded by heat, light or air, the ease of adaptation to confirmatory procedures, the potential for automation and the dramatic improvement in instrumentation, including the development of increasingly sensitive fluorescence and electrochemical detectors and short, high-resolution, reversed-phase columns.
本文综述了几种用于测定农产品和食品中黄曲霉毒素的色谱方法。在过去二十年中，黄曲霉毒素的鉴定和测定是通过薄层色谱法（TLC）完成的，因为它简便、快速且成本低廉。然而，如今采用荧光检测的高效液相色谱法（HPLC）已成为测定黄曲霉毒素的首选方法，并且在黄曲霉毒素鉴定方面也越来越受欢迎。选择HPLC而非TLC的原因可概括为：能够分析多种化合物，包括易受热、光或空气降解的化合物；易于采用确证程序；具有自动化潜力；以及仪器的显著改进，包括开发出越来越灵敏的荧光和电化学检测器以及短的、高分辨率的反相柱。
