Awadallah Samir M, Atoum Manar F
Department of Medical Laboratory Sciences, The Hashemite University, Faculty of Allied Health Sciences, P.O. Box 330077, Zarqa 13133, Jordan.
Clin Chim Acta. 2004 Mar;341(1-2):17-21. doi: 10.1016/j.cccn.2003.10.032.
Previous reports regarding the occurrence of breast cancer and its association with Hp polymorphism are conflicting. The possible role of family history as a factor in determining the degree of association between the disease and Hp polymorphism has not been reported before. In this study, the distribution of haptoglobin phenotype among patients with familial and nonfamilial breast cancer was investigated.
Haptoglobin phenotypes were determined in serum of 128 breast cancer patients (familial, n=42; nonfamilial, n=86) and in controls (n=200) by vertical polyacrylamide gel electrophoresis.
No significant difference of Hp phenotype distribution was observed between patients as a combined group when compared with the control group. In the familial group, the frequency of Hp1-1 and Hp2-1 phenotype distribution was higher and Hp2-2 was lower than that in the nonfamilial and the control groups. Similar but inversed Hp distribution pattern was observed in the nonfamilial group when compared with that in the other groups. An appreciable finding is the observation that Hp2-2 phenotype frequency in the nonfamilial group was significantly higher than that in the familial group (p=0.0365).
Results of this study demonstrate that the pattern of Hp phenotype distribution in breast cancer patients is family history-dependent. Hp1 and Hp2 allele frequencies were over-represented in patients with familial and nonfamilial breast cancer, respectively. The pattern is probably attributed to genetic and oxidative stress mechanisms.
先前关于乳腺癌的发生及其与Hp多态性的关联的报道相互矛盾。家族史作为决定该疾病与Hp多态性之间关联程度的一个因素，其可能的作用此前尚未见报道。在本研究中，对家族性和非家族性乳腺癌患者中触珠蛋白表型的分布进行了调查。
通过垂直聚丙烯酰胺凝胶电泳测定了128例乳腺癌患者（家族性，n = 42；非家族性，n = 86）以及200例对照者血清中的触珠蛋白表型。
与对照组相比，作为一个合并组的患者之间触珠蛋白表型分布未观察到显著差异。在家族性组中，Hp1-1和Hp2-1表型分布的频率较高，而Hp2-2的频率低于非家族性组和对照组。与其他组相比，非家族性组中观察到类似但相反的Hp分布模式。一个值得注意的发现是，非家族性组中Hp2-2表型频率显著高于家族性组（p = 0.0365）。
本研究结果表明，乳腺癌患者中触珠蛋白表型分布模式依赖于家族史。Hp1和Hp2等位基因频率分别在家族性和非家族性乳腺癌患者中过度表达。这种模式可能归因于遗传和氧化应激机制。
