Vellenga L, Egberts H J, Wensing T, van Dijk J E, Mouwen J M, Breukink H J
Clinic for Large Animal Medicine, State University of Utrecht, The Netherlands.
Am J Vet Res. 1992 Jul;53(7):1180-3.
Macromolecular permeability of the small intestine was tested in four 3-week-old gnotobiotic pigs inoculated with porcine rotavirus strain RV277 (group A). Pigs were administered 125I-labeled polyvinylpyrrolidone (molecular weight [mol wt], 40,000) orally 1 day before and 2 and 24 hours after virus inoculation, and blood samples were obtained every 6 hours. Eight hours after rotavirus inoculation, pigs had watery diarrhea. Increased permeation of 125I-labeled polyvinylpyrrolidone was not observed after clinical signs of infection had developed. Serum total protein and urea nitrogen concentrations increased slightly at the end of the study, probably as a consequence of dehydration. Differences in blood glucose concentration were not seen. At 48 hours after viral inoculation, macromolecular permeability was tested morphologically by injecting horseradish peroxidase (mol wt, 40,000) into the jejunal lumen just distally to the ligamentum colicoduodenale. After an incubation period of 20 minutes, small segments of jejunum were obtained for stereomicroscopic, histologic, and ultrastructural investigations. Moderate hyperregenerative villus atrophy was found. Ultrastructural changes of the villus epithelium were minor, and increased macromolecular permeation was not observed.
在4头接种了猪轮状病毒株RV277（A组）的3周龄无菌猪中测试了小肠的大分子通透性。在病毒接种前1天以及接种后2小时和24小时，给猪口服125I标记的聚乙烯吡咯烷酮（分子量[mol wt]，40,000），每6小时采集血样。接种轮状病毒8小时后，猪出现水样腹泻。在出现感染的临床症状后，未观察到125I标记的聚乙烯吡咯烷酮的渗透增加。在研究结束时，血清总蛋白和尿素氮浓度略有增加，这可能是脱水的结果。未观察到血糖浓度的差异。在病毒接种后48小时，通过将辣根过氧化物酶（分子量，40,000）注入空肠腔内距结肠十二指肠韧带远端的位置，对大分子通透性进行形态学测试。孵育20分钟后，获取小肠空肠小段用于立体显微镜、组织学和超微结构研究。发现有中度的高再生性绒毛萎缩。绒毛上皮的超微结构变化较小，未观察到大分子渗透增加。
