Tsukahara Takamitsu, Iwasaki Yoshie, Nakayama Keizo, Ushida Kazunari
Laboratory of Animal Science, Kyoto Prefectural University, Shimogamo, Kyoto 606-8522, Japan.
J Nutr Sci Vitaminol (Tokyo). 2003 Dec;49(6):414-21. doi: 10.3177/jnsv.49.414.
Fructooligosaccharides (FOS) reach the large intestine and are fermented into short-chain fatty acids (SCFA), lactate, and carbon dioxide. As the major energy source for the epithelial cells of the large intestine, n-butyrate stimulates the proliferation of cells as well as mineral and water absorption from the lumen. We examined the effect of dietary FOS supplementation on luminal SCFA production and its influence on the morphometrical variables of mucosa of the large intestine in commercially available pigs. Six weaning piglets were used. After 7 d of adaptation, three pigs were given a test diet containing FOS (10%) ad libitum for 10 d. The other three remained on the basal diet and were used as controls. At the end of the experiment, their large intestines were removed, and the cecum, gyri centripetales, gyri centrifugales, and rectum were separated. The contents of each portion were collected and measured for SCFA concentration, pH, and moisture. A micrometer was used to measure the crypt depth. The numbers of epithelial and mitotic cells in the crypt columns were also counted. The concentration of SCFA was significantly higher in piglets fed FOS than in the controls. The concentration of n-butyrate was markedly stimulated by FOS. The number of epithelial. mitotic, and mucin-containing cells was higher in piglets fed FOS than in the controls. Accordingly, the crypt depth was larger in the FOS-fed piglets. The luminal n-butyrate concentration showed a significantly positive correlation with the crypt depth and the number of epithelial, mitotic, and mucin-containing cells.
低聚果糖（FOS）进入大肠并被发酵成短链脂肪酸（SCFA）、乳酸和二氧化碳。作为大肠上皮细胞的主要能量来源，丁酸盐刺激细胞增殖以及矿物质和水分从肠腔的吸收。我们研究了在市售猪中，日粮添加FOS对肠腔SCFA产生的影响及其对大肠黏膜形态计量学变量的影响。使用了六只断奶仔猪。适应7天后，三只猪自由采食含10% FOS的试验日粮，持续10天。另外三只猪继续采食基础日粮并作为对照。在实验结束时，取出它们的大肠，分离出盲肠、向心回、离心回和直肠。收集各部分内容物，测量SCFA浓度、pH值和水分。用显微镜测量隐窝深度。还对隐窝柱中的上皮细胞和有丝分裂细胞数量进行了计数。采食FOS的仔猪中SCFA浓度显著高于对照组。FOS显著刺激了丁酸盐的浓度。采食FOS的仔猪中上皮细胞、有丝分裂细胞和含黏液细胞的数量高于对照组。因此，采食FOS的仔猪隐窝深度更大。肠腔丁酸盐浓度与隐窝深度以及上皮细胞、有丝分裂细胞和含黏液细胞的数量呈显著正相关。
