Jiang Hongliang, Fang Dufei, Hsiao Benjamin S, Chu Benjamin, Chen Weiliam
Department of Biomedical Engineering, State University of New York at Stony Brook, Stony Brook, New York 11794-2580, USA.
Biomacromolecules. 2004 Mar-Apr;5(2):326-33. doi: 10.1021/bm034345w.
Dextran is soluble in both water and organic solvents, so it could be a versatile biomacromolecule for preparing nanofibrous electrospun membranes by blending with either water-soluble bioactive agents or hydrophobic biodegradable polymers for biomedical applications. We have formulated electrospun dextran membranes, and the effects of various processing parameters on the membrane properties were investigated. It was found that uniform nanofibrous dextran membranes could be formed by using water, DMSO/water, and DMSO/DMF mixtures as solvents through adjusting the processing conditions (solution concentration, voltage, and the distance between the electrode and the collecting plate). When water was used as a solvent, up to 10% (w/w) of bovine serum albumin (BSA) or lysozyme could be directly incorporated into the dextran electrospun membrane without compromising its morphology. No significant effect of the electrospinning process on lysozyme activity was observed. The composite electrospun membranes consisting of poly(D,L-lactide-co-glycolide) (PLGA) and dextran were obtained using DMSO/DMF (50/50, volume ratio) mixture as solvents. For cross-linking the electrospun membrane, dextran was modified by substitution of methacrylate groups at the hydroxyl sites. It was found that the electrospun membranes prepared from methacrylated dextran can be cured by UV irradiation in the presence of 1% of 2,2-dimethoxy-2-phenylacetophenone (DMPA) as a photoinitiator.
右旋糖酐可溶于水和有机溶剂，因此它可能是一种通用的生物大分子，可通过与水溶性生物活性剂或疏水性可生物降解聚合物混合来制备用于生物医学应用的纳米纤维电纺膜。我们已经制备了电纺右旋糖酐膜，并研究了各种加工参数对膜性能的影响。结果发现，通过调节加工条件（溶液浓度、电压以及电极与收集板之间的距离），使用水、二甲基亚砜/水和二甲基亚砜/二甲基甲酰胺混合物作为溶剂可以形成均匀的纳米纤维右旋糖酐膜。当使用水作为溶剂时，高达10%（w/w）的牛血清白蛋白（BSA）或溶菌酶可以直接掺入右旋糖酐电纺膜中而不影响其形态。未观察到电纺过程对溶菌酶活性有显著影响。使用二甲基亚砜/二甲基甲酰胺（50/50，体积比）混合物作为溶剂获得了由聚（D，L-丙交酯-共-乙交酯）（PLGA）和右旋糖酐组成的复合电纺膜。为了交联电纺膜，通过在羟基位点取代甲基丙烯酸酯基团对右旋糖酐进行了改性。结果发现，由甲基丙烯酸化右旋糖酐制备的电纺膜可以在1%的2,2-二甲氧基-2-苯基苯乙酮（DMPA）作为光引发剂的存在下通过紫外线照射进行固化。
