Phukan Anil C, Patgiri Dilip K, Mahanta Jagadish
Regional Medical Research Centre (NE Region), ICMR, Post Box-105, Dibrugarh.
Indian J Pathol Microbiol. 2003 Apr;46(2):274-8.
Faecal specimens from 202 children below 5 years with acute diarrhoea hospitalized in Assam Medical College from April, 1999 to March, 2000 were examined in Regional Medical Research Centre (ICMR), Dibrugarh to know the prevalence of rotavirus diarrhoea and molecular pattern of viral strains from different localities of Dibrugarh using double antibody sandwich ELISA and SDS-PAGE analysis. Human group A rotaviruses were detected in 47 (23.27%) specimens and 33 of 41 (80.49%) positive specimens were electropherotyped where 16 were "long" (48.48%) and 17 "short" (51.52%) types. Rotavirus diarrhoea was significantly high (p<0.01) in children between 11 to 20 months (37.75%). Children from families of upper middle socioeconomic status (61.59%) suffer most (p<0.001). Peak incidence of rotavirus diarrhoea was in winter (38.37%) and showed inverse relation with temperature, humidity and rainfall. Besides diarrhoea, vomiting was a significant clinical manifestation. "Short" electropherotype were common during winter months and in tea garden localities.
1999年4月至2000年3月期间，在阿萨姆医学院住院的202名5岁以下急性腹泻儿童的粪便标本，于迪布鲁格尔的区域医学研究中心（印度医学研究理事会）进行检测，以了解轮状病毒腹泻的患病率以及使用双抗体夹心酶联免疫吸附测定和十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳分析来自迪布鲁格尔不同地区的病毒株分子模式。在47份（23.27%）标本中检测到人类A组轮状病毒，41份阳性标本中的33份（80.49%）进行了电泳分型，其中16份为“长”型（48.48%），17份为“短”型（51.52%）。11至20个月的儿童中轮状病毒腹泻显著高发（p<0.01）（37.75%）。社会经济地位中上层家庭的儿童受影响最大（61.59%）（p<0.001）。轮状病毒腹泻的高发季节为冬季（38.37%），且与温度、湿度和降雨量呈负相关。除腹泻外，呕吐是一个显著的临床表现。“短”电泳型在冬季月份和茶园地区较为常见。
