从印度北方邦住院腹泻儿童中检测轮状病毒。
Detection of rotavirus from hospitalized diarrheic children in uttar pradesh, India.
机构信息
Department of Veterinary Microbiology and Immunology, College of Veterinary Sciences and Animal Husbandry, DUVASU, Mathura, 281001 Uttar Pradesh India ; Division of Virology, Indian Veterinary Research Institute, Izatnagar, Bareilly, 243122 India.
出版信息
Indian J Microbiol. 2012 Sep;52(3):472-7. doi: 10.1007/s12088-012-0279-6. Epub 2012 Jun 22.
In the present study 220 stool samples collected from diarrheic children admitted to different hospitals and nursing homes of Uttar Pradesh and Uttarakhand were screened for rotavirus. Of 220 diarrheic samples screened 46 samples were found to be positive for rotavirus by RNA PAGE. All the isolates exhibited 4-2-3-2 migration pattern suggesting group A rotavirus. Both long and short electropherotypes were prevalent in these regions. Six different electropherotypes were detected in this study period. Male diarrheic children were found to be more susceptible to rotavirus infection (22.96 %) than that of the female ones (17.64 %). Viral RNA isolated from stool samples again subjected to VP4 gene amplification by RT-PCR using con2 and con3 primer which resulted 876 bp product suggesting group A rotavirus. Besides virus isolation was successfully done using MA104 cell line.
在本研究中，从北方邦和北阿坎德邦的不同医院和疗养院收集了 220 份腹泻儿童的粪便样本，对其进行了轮状病毒筛查。在筛查的 220 份腹泻样本中，有 46 份通过 RNA PAGE 检测出轮状病毒呈阳性。所有分离株均表现出 4-2-3-2 迁移模式，提示为 A 组轮状病毒。在这些地区，长型和短型电泳型均很普遍。在此研究期间共检测到 6 种不同的电泳型。与女性（17.64%）相比，男性腹泻儿童更容易受到轮状病毒感染（22.96%）。从粪便样本中分离出的病毒 RNA 再次通过 RT-PCR 使用 con2 和 con3 引物对 VP4 基因进行扩增，得到 876bp 的产物，提示为 A 组轮状病毒。此外，还成功使用 MA104 细胞系进行了病毒分离。
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