Vaneechoutte Mario, Kämpfer Peter, De Baere Thierry, Falsen Enevold, Verschraegen Gerda
Department of Clinical Chemistry, Microbiology and Immunology, Blok A, Ghent University Hospital, De Pintelaan 185, B-9000 Ghent, Belgium.
Institut für Angewandte Mikrobiologie, Justus Liebig Universität, Giessen, Germany.
Int J Syst Evol Microbiol. 2004 Mar;54(Pt 2):317-327. doi: 10.1099/ijs.0.02754-0.
Comparative 16S rDNA sequence analysis indicates that two distinct sublineages, with a sequence dissimilarity of >4 % (bootstrap value, 100 %), exist within the genus RALSTONIA: the Ralstonia eutropha lineage, which comprises Ralstonia basilensis, Ralstonia campinensis, R. eutropha, Ralstonia gilardii, Ralstonia metallidurans, Ralstonia oxalatica, Ralstonia paucula, Ralstonia respiraculi and Ralstonia taiwanensis; and the Ralstonia pickettii lineage, which comprises Ralstonia insidiosa, Ralstonia mannitolilytica, R. pickettii, Ralstonia solanacearum and Ralstonia syzygii comb. nov. (previously Pseudomonas syzygii). This phylogenetic discrimination is supported by phenotypic differences. Members of the R. eutropha lineage have peritrichous flagella, do not produce acids from glucose and are susceptible to colistin, in contrast to members of the R. pickettii lineage, which have one or more polar flagella, produce acid from several carbohydrates and are colistin-resistant. Members of the R. pickettii lineage are viable for up to 6 days on tryptic soy agar at 25 degrees C, whereas members of the R. eutropha lineage are viable for longer than 9 days. It is proposed that species of the R. eutropha lineage should be classified in a novel genus, Wautersia gen. nov. Finally, based on the literature and new DNA-DNA hybridization data, it is proposed that Pseudomonas syzygii should be renamed Ralstonia syzygii comb. nov.
16S rDNA序列比较分析表明，在罗尔斯通氏菌属内存在两个不同的亚谱系，其序列差异>4%（自展值为100%）：嗜麦芽寡养单胞菌谱系，包括罗尔斯通氏菌属（Ralstonia basilensis）、罗尔斯通氏菌属（Ralstonia campinensis）、嗜麦芽寡养单胞菌（R. eutropha）、罗尔斯通氏菌属（Ralstonia gilardii）、嗜金属罗尔斯通氏菌（Ralstonia metallidurans）、草酸罗尔斯通氏菌（Ralstonia oxalatica）、少动罗尔斯通氏菌（Ralstonia paucula）、呼吸罗尔斯通氏菌（Ralstonia respiraculi）和台湾罗尔斯通氏菌（Ralstonia taiwanensis）；以及皮氏罗尔斯通氏菌谱系，包括罗尔斯通氏菌属（Ralstonia insidiosa）、甘露醇罗尔斯通氏菌（Ralstonia mannitolilytica）、皮氏罗尔斯通氏菌（R. pickettii）、茄科劳尔氏菌（Ralstonia solanacearum）和新组合的合柱罗尔斯通氏菌（Ralstonia syzygii comb. nov.）（以前的合柱假单胞菌（Pseudomonas syzygii））。这种系统发育上的区分得到了表型差异的支持。嗜麦芽寡养单胞菌谱系的成员有周生鞭毛，不能从葡萄糖产酸，对黏菌素敏感，与之形成对比的是，皮氏罗尔斯通氏菌谱系的成员有一根或多根极生鞭毛，能从几种碳水化合物产酸，对黏菌素耐药。皮氏罗尔斯通氏菌谱系的成员在25℃的胰蛋白胨大豆琼脂上可存活长达6天，而嗜麦芽寡养单胞菌谱系的成员可存活超过9天。建议将嗜麦芽寡养单胞菌谱系的物种归入一个新属，即沃氏菌属（Wautersia gen. nov.）。最后，根据文献和新的DNA-DNA杂交数据，建议将合柱假单胞菌重新命名为新组合的合柱罗尔斯通氏菌（Ralstonia syzygii comb. nov.）。
