Brüstle O, Spiegel H, Leib S L, Finn T, Stein H, Kleihues P, Wiestler O D
Department of Pathology, University of Zürich, Switzerland.
Acta Neuropathol. 1992;84(1):24-31. doi: 10.1007/BF00427211.
The presence and distribution of human immunodeficiency virus (HIV) were examined in the CNS of two children with severe HIV encephalitis and myelitis. Using polymerase chain reaction-mediated DNA amplification and subsequent Southern analysis, proviral HIV gag sequences were identified in brain tissue of both patients. In situ hybridization using antisense oligonucleotide probes revealed abundant HIV gag and env/nef RNAs selectively in areas with histopathological evidence for HIV-induced tissue damage. The spinal cord of one patient exhibited a striking subpial accumulation of HIV RNAs strongly suggestive of a liquorigenic spread of the infection. HIV RNAs were typically associated with cells of the monocyte/macrophage lineage, as shown by a combined immunohistochemical and in situ hybridization procedure. The present study supports the view that the pattern and distribution of HIV-induced brain lesions is largely determined by the extent of focal HIV replication within the CNS.
对两名患有严重人类免疫缺陷病毒（HIV）脑炎和脊髓炎的儿童的中枢神经系统（CNS）进行了HIV的存在情况及分布检查。利用聚合酶链反应介导的DNA扩增及后续的Southern分析，在两名患者的脑组织中均鉴定出了前病毒HIV gag序列。使用反义寡核苷酸探针进行的原位杂交显示，在有HIV诱导组织损伤的组织病理学证据的区域中，选择性地存在丰富的HIV gag和env/nef RNA。一名患者的脊髓显示出HIV RNA在软膜下显著积聚，强烈提示感染通过脑脊液扩散。如免疫组织化学和原位杂交联合检测所示，HIV RNA通常与单核细胞/巨噬细胞谱系的细胞相关。本研究支持这样一种观点，即HIV诱导的脑损伤的模式和分布在很大程度上由CNS内局部HIV复制的程度决定。
