Goto Y, O'Malley C, Fanning N F, Wang J, Redmond H P, Shorten G D
Intensive Care Unit, Sir Charles Gairdner Hospital, Nedlands, WA, Australia.
Ir J Med Sci. 2003 Oct-Dec;172(4):191-4. doi: 10.1007/BF02915288.
Benzodiazepines, which are commonly administered perioperatively, can depress immune function. Neutrophil apoptosis plays a central role in the regulation of inflammation. This is particularly important during and after surgery.
To examine the effects of benzodiazepines (midazolam and diazepam) on neutrophil apoptosis.
Venous blood samples were withdrawn from patients scheduled to undergo elective surgery, (a) immediately prior to, and 10 minutes after administration of midazolam 0.2 mg/kg intravenously (n=11) and (b) immediately prior to, and 60 minutes after administration of diazepam 10 mg p.o. (n=10). Neutrophil apoptosis was measured by Annexin V-FITC after 1 and 12 hours in culture.
The percentage of apoptotic cells was significantly less after midazolam at 12% (11.9) hours in culture compared to pre-midazolam 29.7% (13.3) (p<0.05). After diazepam, the rates of neutrophil apoptosis were also significantly less after 12 hours in culture (p<0.05).
Administration of benzodiazepines in clinically relevant doses inhibits neutrophil apoptosis. In the perioperative period, this may influence the inflammatory response to surgery.
苯二氮䓬类药物常用于围手术期，可抑制免疫功能。中性粒细胞凋亡在炎症调节中起核心作用。这在手术期间及术后尤为重要。
研究苯二氮䓬类药物（咪达唑仑和地西泮）对中性粒细胞凋亡的影响。
从计划接受择期手术的患者中采集静脉血样本，(a)静脉注射0.2 mg/kg咪达唑仑前及注射后10分钟（n = 11），(b)口服10 mg地西泮前及服药后60分钟（n = 10）。培养1小时和12小时后，采用膜联蛋白V-异硫氰酸荧光素法检测中性粒细胞凋亡。
与咪达唑仑给药前的29.7%（13.3）相比，培养12小时后咪达唑仑组凋亡细胞百分比显著降低，为12%（11.9）（p < 0.05）。地西泮给药后，培养12小时后中性粒细胞凋亡率也显著降低（p < 0.05）。
临床相关剂量的苯二氮䓬类药物给药可抑制中性粒细胞凋亡。在围手术期，这可能会影响对手术的炎症反应。
