Humblot Christèle, Lhoste Evelyne, Knasmüller Siegfried, Gloux Karine, Bruneau Aurélia, Bensaada Martine, Durao José, Rabot Sylvie, Andrieux Claude, Kassie Fekadu
Institut National de la Recherche Agronomique, Unité d'Ecologie et de Physiologie du Système Digestif, Bâtiment Jacques Poly, 78352 Jouy-en-Josas, France.
J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Mar 25;802(1):231-7. doi: 10.1016/j.jchromb.2003.11.018.
We investigated the chemoprotective effects of four common constituents of the human diet, i.e. a fermented milk, inulin, oligofructose and Brussels sprouts, towards 2-amino-3-methylimidazo[4,5-f]quinoline (IQ)-induced genotoxicity in male Fischer 344 rats harbouring a human intestinal microflora. We found that the four dietary components significantly reduced IQ-induced DNA damage in hepatocytes (reduction ranged from 74% with inulin to 39% with Brussels sprouts) and colonocytes (reduction ranged from 68% with inulin to 56% with Brussels sprouts). This chemoprotective effect correlated with the induction of hepatic UDP-glucuronosyl transferase following Brussels sprouts consumption, and with alterations of bacterial metabolism in the distal gut (acidification, increase of butyrate proportion, decrease of beta-glucuronidase activity) following inulin consumption.
