白色念珠菌的SKN7：突变体构建与表型分析。
SKN7 of Candida albicans: mutant construction and phenotype analysis.
作者信息
Singh Praveen, Chauhan Neeraj, Ghosh Anup, Dixon Freddie, Calderone Richard
机构信息
Department of Microbiology and Immunology, Georgetown University Medical Center, Washington, DC 20007, USA.
出版信息
Infect Immun. 2004 Apr;72(4):2390-4. doi: 10.1128/IAI.72.4.2390-2394.2004.
Abstract
The SKN7 two-component response regulator gene of Candida albicans was deleted, and the phenotype of the mutant was established. This mutant exhibited impaired growth on Spider agar and 10% serum agar compared to wild-type and gene-reconstituted strains. The skn7 mutant was sensitive to H(2)O(2) in vitro, but its virulence was only mildly attenuated. A comparison of the Skn7p and Ssk1p response regulators of C. albicans is discussed.
摘要
白色念珠菌的双组分反应调节基因SKN7被敲除，并确定了突变体的表型。与野生型和基因重组菌株相比，该突变体在蜘蛛琼脂和10%血清琼脂上生长受损。skn7突变体在体外对H₂O₂敏感，但其毒力仅轻度减弱。文中讨论了白色念珠菌的Skn7p和Ssk1p反应调节因子的比较。
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